Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (ab183721)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR7226(2)] to Aconitase 1/ACO1
Suitable for: WB
Knockout validated
Reacts with: Human

Product name

Anti-Aconitase 1/ACO1 antibody [EPR7226(2)]
See all Aconitase 1/ACO1 primary antibodies
Description

Rabbit monoclonal [EPR7226(2)] to Aconitase 1/ACO1
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human fetal liver tissue lysate, HepG2, T.T, U20S, HeLa and HEK-293 whole cell lysate
General notes
This product was previously labelled as Aconitase 1

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR7226(2)
Isotype

IgG
Research areas

Western blot - Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (ab183721)

All lanes : Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (ab183721) at 1 µg/ml

Lane 1 : Wild-type U20S whole cell lysate
Lane 2 : ACO1 knockout U20S whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : HEK-293 whole cell lysate

Lysates/proteins at 20 µg per lane.

Predicted band size: 98 kDa

Lanes 1 - 4: Merged signal (red and green). Green - ab183721 observed at 98 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab183721 was shown to specifically react with ACO1 in wild-type U20S cells as signal was lost in ACO1 knockout cells. Wild-type and ACO1 knockout samples were subjected to SDS-PAGE. Ab183721 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye^® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (ab183721)

Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (ab183721) at 1/20000 dilution + Human fetal liver tissue lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 98 kDa
Western blot - Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (ab183721)

All lanes : Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (ab183721) at 1/1000 dilution

Lane 1 : HepG2 cell lysate
Lane 2 : T.T cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/5000 dilution

Predicted band size: 98 kDa
Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (ab183721)

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