Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (ab183721)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7226(2)] to Aconitase 1/ACO1
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Aconitase 1/ACO1 antibody [EPR7226(2)]
    See all Aconitase 1/ACO1 primary antibodies

  • Description

    Rabbit monoclonal [EPR7226(2)] to Aconitase 1/ACO1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal liver tissue lysate, HepG2, T.T, U20S, HeLa and HEK-293 whole cell lysate

  • General notes

     

     

     This product was previously labelled as Aconitase 1

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (ab183721)
    Western blot - Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (ab183721)
    All lanes : Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (ab183721) at 1 µg/ml

    Lane 1 : Wild-type U20S whole cell lysate
    Lane 2 : ACO1 knockout U20S whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : HEK-293 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 98 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab183721 observed at 98 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab183721 was shown to specifically react with ACO1 in wild-type U20S cells as signal was lost in ACO1 knockout cells. Wild-type and ACO1 knockout samples were subjected to SDS-PAGE. Ab183721 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (ab183721)
    Western blot - Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (ab183721)
    Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (ab183721) at 1/20000 dilution + Human fetal liver tissue lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 98 kDa

  • Western blot - Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (ab183721)
    Western blot - Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (ab183721)
    All lanes : Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (ab183721) at 1/1000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : T.T cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/5000 dilution

    Predicted band size: 98 kDa

  • Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (ab183721)
    Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (ab183721)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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