Anti-SLCO2B1/OATP2B1 antibody [EPR22212] (ab222094)
![Anti-SLCO2B1/OATP2B1 antibody [EPR22212] (ab222094)](https://www.abcam.com/ps/products/222/ab222094/Images/ab222094-323597-anti-slco2b1-antibody-epr22212-immunohistochemistry.jpg "Anti-SLCO2B1/OATP2B1 antibody [EPR22212] (ab222094)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22212] to SLCO2B1/OATP2B1
- Suitable for: IHC-P, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-SLCO2B1/OATP2B1 antibody [EPR22212]
See all SLCO2B1/OATP2B1 primary antibodies -
Description
Rabbit monoclonal [EPR22212] to SLCO2B1/OATP2B1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human liver and placenta tissue. Flow Cyt: Huh7 cells.
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General notes
This product was previously labelled as SLCO2B1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22212 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLCO2B1/OATP2B1 antibody [EPR22212] (ab222094)
Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling SLCO2B1/OATP2B1 with ab222094 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Basolateral membranous staining on human placenta (PMID: 17020956; PMID: 20159975) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Flow Cytometry - Anti-SLCO2B1/OATP2B1 antibody [EPR22212] (ab222094)](https://www.abcam.com/ps/products/222/ab222094/Images/ab222094-323598-anti-slco2b1-antibody-epr22212-flow-cytometry.jpg)
Flow Cytometry - Anti-SLCO2B1/OATP2B1 antibody [EPR22212] (ab222094)Flow cytometric analysis of Huh7 (human hepatocellular carcinoma epithelial cell line) cells labeling SLCO2B1/OATP2B1 with ab222094 at 1/50 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLCO2B1/OATP2B1 antibody [EPR22212] (ab222094)](https://www.abcam.com/ps/products/222/ab222094/Images/ab222094-323596-anti-slco2b1-antibody-epr22212-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLCO2B1/OATP2B1 antibody [EPR22212] (ab222094)Immunohistochemical analysis of paraffin-embedded human liver tissue labeling SLCO2B1/OATP2B1 with ab222094 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous staining on human liver (PMID: 17178262; PMID: 11159893; PMID: 23987090) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Anti-SLCO2B1/OATP2B1 antibody [EPR22212] (ab222094)](https://www.abcam.com/ps/products/222/ab222094/Images/ab222094-4-benefits-of-recombinant-antibodies.png)
Anti-SLCO2B1/OATP2B1 antibody [EPR22212] (ab222094)
