Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FMO3 antibody [EPR6968] (ab126711)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6968] to FMO3
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-FMO3 antibody [EPR6968]
    See all FMO3 primary antibodies

  • Description

    Rabbit monoclonal [EPR6968] to FMO3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Mouse
    Rat
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human FMO3 aa 300-400. The exact sequence is proprietary.
    Database link: P31513

  • Positive control

    • WB: Human fetal liver, human fetal kidney, mouse kidney, mouse liver, rat kidney and rat liver tissue lysates. IHC-P: Human, mouse and rat liver tissues. ICC/IF: HepG2 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-FMO3 antibody [EPR6968] (ab126711)
    Western blot - Anti-FMO3 antibody [EPR6968] (ab126711)
    All lanes : Anti-FMO3 antibody [EPR6968] (ab126711) at 1/5000 dilution (purified)

    Lane 1 : Human fetal kidney tissue lysate
    Lane 2 : Human fetal liver tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 60 kDa
    Observed band size: 56 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer: 5% NFDM/TBST.

  • Western blot - Anti-FMO3 antibody [EPR6968] (ab126711)
    Western blot - Anti-FMO3 antibody [EPR6968] (ab126711)
    All lanes : Anti-FMO3 antibody [EPR6968] (ab126711) at 1/5000 dilution (purified)

    Lane 1 : Mouse kidney tissue lysate
    Lane 2 : Mouse liver tissue lysate
    Lane 3 : Rat kidney tissue lysate
    Lane 4 : Rat liver tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 60 kDa
    Observed band size: 56 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer: 5% NFDM/TBST.

  • Western blot - Anti-FMO3 antibody [EPR6968] (ab126711)
    Western blot - Anti-FMO3 antibody [EPR6968] (ab126711)
    All lanes : Anti-FMO3 antibody [EPR6968] (ab126711) at 1/1000 dilution (unpurified)

    Lane 1 : Human fetal liver tissue lysate
    Lane 2 : Human fetal kidney tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 60 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FMO3 antibody [EPR6968] (ab126711)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FMO3 antibody [EPR6968] (ab126711)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling FMO3 with purified ab126711 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FMO3 antibody [EPR6968] (ab126711)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FMO3 antibody [EPR6968] (ab126711)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue labelling FMO3 with purified ab126711 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FMO3 antibody [EPR6968] (ab126711)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FMO3 antibody [EPR6968] (ab126711)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue labelling FMO3 with purified ab126711 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FMO3 antibody [EPR6968] (ab126711)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FMO3 antibody [EPR6968] (ab126711)

    Immunohistochemitsy (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling FMO3 with unpurified ab126711 at a 1/500 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-FMO3 antibody [EPR6968] (ab126711)
    Immunocytochemistry/ Immunofluorescence - Anti-FMO3 antibody [EPR6968] (ab126711)

    Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labelling FMO3 with purified ab126711 at 1/150. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/500) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500) were also used.

    Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

  • OI-RD Scanning - Anti-FMO3 antibody [EPR6968] (ab126711)
    OI-RD Scanning - Anti-FMO3 antibody [EPR6968] (ab126711)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-FMO3 antibody [EPR6968] (ab126711)
    Anti-FMO3 antibody [EPR6968] (ab126711)

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