Antibodies, Proteins, Kits and Reagents for Life Science

Anti-alpha smooth muscle Actin antibody [ACTA2/791] (ab220179)

Key features and details

Mouse monoclonal [ACTA2/791] to alpha smooth muscle Actin
Suitable for: IHC-P, ICC
Reacts with: Rat, Human
Isotype: IgG2a

Product name

Anti-alpha smooth muscle Actin antibody [ACTA2/791]
See all alpha smooth muscle Actin primary antibodies
Description

Mouse monoclonal [ACTA2/791] to alpha smooth muscle Actin
Host species

Mouse
Tested Applications & Species

Application Species
IHC-P
Rat

Human

See all applications and species data
Immunogen

Recombinant full length protein corresponding to Human alpha smooth muscle Actin.
Database link: P62736
Positive control
- IHC: Human angiosarcoma, leiomyosarcoma, colon carcinoma and rat lung tissues. ICC: HeLa cells.
General notes

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Application	Species
IHC-P	Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA
Concentration information loading...
Purity

Protein A/G purified
Purification notes

ab220179 was purified from Bioreactor Concentrate by Protein A/G.
Clonality

Monoclonal
Clone number

ACTA2/791
Isotype

IgG2a
Light chain type

kappa
Research areas

Immunocytochemistry - Anti-alpha smooth muscle Actin antibody [ACTA2/791] (ab220179)

Immunofluorescence Analysis of HeLa cells labeling Smooth Muscle Actin with ab220179 followed by Goat anti-Mouse IgG-CF488(Green). The nuclear counterstain is NucSpot (Red).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [ACTA2/791] (ab220179)

Immunohistochemical analysis of formalin fixed, paraffin embedded Human angiosarcoma tissue labeling alpha smooth muscle Actin with ab220179 at 0.5 µg/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [ACTA2/791] (ab220179)

Immunohistochemical analysis of formalin fixed, paraffin embedded Human leiomyosarcoma tissue labeling alpha smooth muscle Actin with ab220179 at 0.5 µg/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [ACTA2/791] (ab220179)

Immunohistochemical analysis of formalin fixed, paraffin embedded Human colon carcinoma tissue labeling alpha smooth muscle Actin with ab220179 at 0.5 µg/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [ACTA2/791] (ab220179)

Immunohistochemical analysis of formalin fixed, paraffin embedded Rat lung tissue labeling alpha smooth muscle Actin with ab220179 at 0.5 µg/ml.

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