Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PTGR1 antibody [EPR13451-10] (ab181131)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13451-10] to PTGR1
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-PTGR1 antibody [EPR13451-10]
    See all PTGR1 primary antibodies

  • Description

    Rabbit monoclonal [EPR13451-10] to PTGR1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HT-29 and HeLa cell lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PTGR1 antibody [EPR13451-10] (ab181131)
    Western blot - Anti-PTGR1 antibody [EPR13451-10] (ab181131)
    All lanes : Anti-PTGR1 antibody [EPR13451-10] (ab181131) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : PTGR1 knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 35 kDa
    Observed band size: 38 kDa
    why is the actual band size different from the predicted?



    Lanes 1-2: Merged signal (red and green). Green - ab181131 observed at 38 kDa. Red - loading control ab7291 observed at 50 kDa.

     ab181131 Anti-PTGR1 antibody [EPR13451-10] was shown to specifically react with PTGR1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265169 (knockout cell lysate ab258152) was used. Wild-type and PTGR1 knockout samples were subjected to SDS-PAGE. ab181131 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-PTGR1 antibody [EPR13451-10] (ab181131)
    Western blot - Anti-PTGR1 antibody [EPR13451-10] (ab181131)
    Anti-PTGR1 antibody [EPR13451-10] (ab181131) at 1/2000 dilution + HT-29 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 35 kDa
    Observed band size: 36 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-PTGR1 antibody [EPR13451-10] (ab181131)
    Western blot - Anti-PTGR1 antibody [EPR13451-10] (ab181131)
    Anti-PTGR1 antibody [EPR13451-10] (ab181131) at 1/1000 dilution + HeLa cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 35 kDa
    Observed band size: 36 kDa why is the actual band size different from the predicted?

  • Anti-PTGR1 antibody [EPR13451-10] (ab181131)
    Anti-PTGR1 antibody [EPR13451-10] (ab181131)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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