Astana Biomed Group, an authorized Abcam distributor in Central Asia

PE Anti-alpha smooth muscle Actin antibody [E184] (ab209435)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • PE Rabbit monoclonal [E184] to alpha smooth muscle Actin
  • Suitable for: Flow Cyt, ICC/IF
  • Reacts with: Rat, Human
  • Conjugation: PE. Ex: 488nm, Em: 575nm

Overview

  • Product name

    PE Anti-alpha smooth muscle Actin antibody [E184]
    See all alpha smooth muscle Actin primary antibodies

  • Description

    PE Rabbit monoclonal [E184] to alpha smooth muscle Actin

  • Host species

    Rabbit

  • Conjugation

    PE. Ex: 488nm, Em: 575nm

  • Tested applications

    Suitable for: Flow Cyt, ICC/IFmore details

  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse

  • Immunogen

    Synthetic peptide within Human alpha smooth muscle Actin (N terminal). The exact sequence is proprietary.

  • Positive control

    • Flow Cyt: SV40LT-SMC cells ICC/IF: SV40LT-SMC cells

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - PE Anti-alpha smooth muscle Actin antibody [E184] (ab209435)
    Flow Cytometry - PE Anti-alpha smooth muscle Actin antibody [E184] (ab209435)

    Overlay histogram showing SV40LT-SMC cells stained with ab209435 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab209435, 1/5000 dilution) for 30 min at 22ºC.

    Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 20 mW Solid State Blue Laser (488nm) and 585/40 bandpass filter.

    This antibody gave a positive signal in SV40LT-SMC cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

  • Immunocytochemistry/ Immunofluorescence - PE Anti-alpha smooth muscle Actin antibody [E184] (ab209435)
    Immunocytochemistry/ Immunofluorescence - PE Anti-alpha smooth muscle Actin antibody [E184] (ab209435)

    ab209435 staining alpha smooth muscle Actin in SV40LT-SMC cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab209435 at 1/500 dilution (Pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • PE Anti-alpha smooth muscle Actin antibody [E184] (ab209435)
    PE Anti-alpha smooth muscle Actin antibody [E184] (ab209435)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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