Goat Anti-Chicken IgY H&L (HRP) (ab97135)
Key features and details
- Goat Anti-Chicken IgY H&L (HRP)
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC, IHC-P, ELISA, WB
Overview
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Product name
Goat Anti-Chicken IgY H&L (HRP)
See all IgY secondary antibodies -
Host species
Goat -
Target species
Chicken -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Chicken IgY and with light chains common to other Chicken immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. -
Tested applications
Suitable for: ICC, IHC-P, ELISA, WBmore details -
Conjugation
HRP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP). -
Conjugation notes
Molar enzyme/ antibody protein ratio is 4:1 -
Clonality
Polyclonal -
Isotype
IgG -
General notes
Part of the AbExcel range. -
Research areas
Images
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Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab97135)All lanes : Anti-beta Actin antibody - Loading Control (ab13822) at 1 µg/ml
Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.
Secondary
Lanes 1 - 2 : Goat polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab97135) at 1/2000 dilution
Lanes 3 - 4 : Goat polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab97135) at 1/10000 dilution
Lanes 5 - 6 : Goat polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab97135) at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time : 90 seconds -
Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab97135) This image is courtesy of an anonymous Abreview.All lanes : Anti-Vimentin antibody (ab24525)
All lanes : Mouse cardiac fibroblasts
Lysates/proteins at 4 µg per lane.
Secondary
All lanes : Goat Anti-Chicken IgY H&L (HRP) (ab97135) at 1/2500 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 2 seconds
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Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab97135)Ab97135 used to detect a chicken anti-beta Galactosidase antibody (ab9361). Ab97135 used at a concentration of 1:2000 in 5% milk blocking buffer in 0.1% Tween, 1x PBS. Whole lysates from DH5a (E.coli). Specific band at approx. 110 KDa. Non-specific bands: 70 Kda. NB. Gels run on 10% Acrylamide gels.
This image is courtesy of an anonymous Abreview.