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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Actin

Anti-Actin antibody [EPR16770] (ab198991)

Price and availability

291 484 ₸

Availability

Order now and get it on Tuesday March 16, 2021

Anti-Actin antibody [EPR16770] (ab198991)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16770] to Actin
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-Actin antibody [EPR16770]
    See all Actin primary antibodies
  • Description

    Rabbit monoclonal [EPR16770] to Actin
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data
  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, 293, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Human fetal brain, fetal heart, fetal kidney and fetal spleen lysates; Mouse brain, heart, kidney and spleen lysates; Rat brain, heart, kidney and spleen lysates. IP: 293 whole cell extract.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR16770
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Cytoskeleton
    • Actin
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Troponin
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Tropomyosin
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Crosslinking
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Binding Proteins
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Assembly
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Death receptors & ligands
    • RIP
    • Cancer
    • Cell Death
    • Apoptosis
    • Receptors
    • Death receptors & ligands
    • RIP

Images

  • Western blot - Anti-Actin antibody [EPR16770] (ab198991)
    Western blot - Anti-Actin antibody [EPR16770] (ab198991)
    All lanes : Anti-Actin antibody [EPR16770] (ab198991) at 1/20000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
    Lane 2 : 293 (Human embryonic kidney) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    This antibody can recognize ACTS, ACTH, ACTG, ACTC, ACTB & ACTA, it is a pan Actin.

  • Western blot - Anti-Actin antibody [EPR16770] (ab198991)
    Western blot - Anti-Actin antibody [EPR16770] (ab198991)
    All lanes : Anti-Actin antibody [EPR16770] (ab198991) at 1/20000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human fetal heart lysate
    Lane 3 : Human fetal kidney lysate
    Lane 4 : Human fetal spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    This antibody can recognize ACTS, ACTH, ACTG, ACTC, ACTB & ACTA, it is a pan Actin.

  • Western blot - Anti-Actin antibody [EPR16770] (ab198991)
    Western blot - Anti-Actin antibody [EPR16770] (ab198991)
    All lanes : Anti-Actin antibody [EPR16770] (ab198991) at 1/20000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Mouse kidney lysate
    Lane 4 : Mouse spleen lysate
    Lane 5 : Rat brain lysate
    Lane 6 : Rat heart lysate
    Lane 7 : Rat kidney lysate
    Lane 8 : Rat spleen lysate
    Lane 9 : C6 (Rat glial tumor cells) whole cell lysate
    Lane 10 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 11 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 12 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    This antibody can recognize ACTS, ACTH, ACTG, ACTC, ACTB & ACTA, it is a pan Actin.

  • Immunoprecipitation - Anti-Actin antibody [EPR16770] (ab198991)
    Immunoprecipitation - Anti-Actin antibody [EPR16770] (ab198991)

    Actin was immunoprecipitated from 1mg of 293 (Human embryonic kidney) whole cell extract with ab198991 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab198991 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: 293 whole cell extract 10 µg (Input). Lane 2: ab198991 IP in 293 whole cell extract. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab198991 in 293 whole cell extract.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

  • Anti-Actin antibody [EPR16770] (ab198991)
    Anti-Actin antibody [EPR16770] (ab198991)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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