Anti-ATF2 (phospho T71 + T53) antibody (ab28812)
Key features and details
- Rabbit polyclonal to ATF2 (phospho T71 + T53)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ATF2 (phospho T71 + T53) antibody
See all ATF2 primary antibodies -
Description
Rabbit polyclonal to ATF2 (phospho T71 + T53) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthesized phospho peptide derived from human ATF2 around the phosphorylation site of Threonine 71 or 53.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF2 (phospho T71 + T53) antibody (ab28812)Ab28812, at a dilution of 1/50, staining ATF2 in paraffin embedded human breast carcinoma tissue by Immunohistochemistry.
Left image: using the ATF2 antibody
Right image: using the same antibody preincubated with synthesized phospho peptide. -
Western blot - Anti-ATF2 (phospho T71 + T53) antibody (ab28812)All lanes : Anti-ATF2 (phospho T71 + T53) antibody (ab28812) at 1/1000 dilution
Lane 1 : untreated HeLa cells (5-30ug lysate).
Lane 2 : HeLa cells treated with UV light (5-30ug lysate).
Predicted band size: 52 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
