Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ATF2 antibody [E242] (ab32061)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E242] to ATF2
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-ATF2 antibody [E242]
    See all ATF2 primary antibodies

  • Description

    Rabbit monoclonal [E242] to ATF2

  • Host species

    Rabbit

  • Specificity

    ab32061 recognises ATF-2 (activating transcription factor-2, CRE-BP1). It does not cross-react with other ATF family members

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human ATF2 aa 50-150 (N terminal). The exact sequence is proprietary.

  • Positive control

    • WB: HeLa whole cell lysate; IHC-P: Human lung cancer.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF2 antibody [E242] (ab32061)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF2 antibody [E242] (ab32061)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung cancer tissue labeling ATF2 with ab32061 at 1/100 dilution (2.55 µg/mL). Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9 (epitope retrieval solution 2). Positive staining on lung cancer. The section was incubated with ab32061 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument and visualised using ab209101, Rabbit specific IHC polymer detection kit HRP/DAB. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Western blot - Anti-ATF2 antibody [E242] (ab32061)
    Western blot - Anti-ATF2 antibody [E242] (ab32061)
    Anti-ATF2 antibody [E242] (ab32061) at 1/10000 dilution + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 54 kDa
    Observed band size: 70 kDa
    why is the actual band size different from the predicted?


    Exposure time: 90 seconds


    Blocking buffer and concentration: 5% NFDM/TBST

  • Immunoprecipitation - Anti-ATF2 antibody [E242] (ab32061)
    Immunoprecipitation - Anti-ATF2 antibody [E242] (ab32061)

    ab32061 (purified) at 1/30 dilution immunoprecipitating ATF2 in HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate.
    Lane 1 (input): HeLa whole cell lysate 10µg
    Lane 2 (+): ab32061 + HeLa whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32061 in HeLa whole cell lysate

    For western blotting, ab32061 at 1/500 and VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1000 dilution. 

    Blocking and diluting buffer: 5% NFDM/TBST.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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