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Epigenetics and Nuclear Signaling Transcription Domain Families HLH / Leucine Zipper Leucine Zipper

Anti-Sacsin antibody [EPR11864-61] (ab181207)

Price and availability

291 484 ₸

Availability

Order now and get it on Friday March 12, 2021

Anti-Sacsin antibody [EPR11864-61] (ab181207)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11864-61] to Sacsin
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human

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Overview

  • Product name

    Anti-Sacsin antibody [EPR11864-61]
    See all Sacsin primary antibodies
  • Description

    Rabbit monoclonal [EPR11864-61] to Sacsin
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal brain, Human glioma and HeLa cell lysates; HeLa cells.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR11864-61
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HLH / Leucine Zipper
    • Leucine Zipper
    • Signal Transduction
    • Protein Trafficking
    • Chaperones
    • Other Chaperones
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers

Images

  • Flow Cytometry - Anti-Sacsin antibody [EPR11864-61] (ab181207)
    Flow Cytometry - Anti-Sacsin antibody [EPR11864-61] (ab181207)

    Flow cytometric analysis of HeLa cells (2% paraformaldehyde-fixed) labeling Sacsin with ab181207 at 1/470 dilution (red), or a rabbit IgG isotype control (green). Goat anti rabbit IgG (FITC) was used as a secondary antibody at 1/150 dilution.

  • Western blot - Anti-Sacsin antibody [EPR11864-61] (ab181207)
    Western blot - Anti-Sacsin antibody [EPR11864-61] (ab181207)
    All lanes : Anti-Sacsin antibody [EPR11864-61] (ab181207) at 1/5000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human glioma cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 521 kDa



    Blocking/diluting buffer and concentration: 5% NFDM/TBST

  • Western blot - Anti-Sacsin antibody [EPR11864-61] (ab181207)
    Western blot - Anti-Sacsin antibody [EPR11864-61] (ab181207)
    Anti-Sacsin antibody [EPR11864-61] (ab181207) at 1/20000 dilution + HeLa cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 521 kDa

  • Anti-Sacsin antibody [EPR11864-61] (ab181207)
    Anti-Sacsin antibody [EPR11864-61] (ab181207)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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