Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Sacsin antibody (ab80195)

Key features and details

  • Rabbit polyclonal to Sacsin
  • Suitable for: ICC/IF, IHC-P
  • Reacts with: Rat, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Sacsin antibody
    See all Sacsin primary antibodies

  • Description

    Rabbit polyclonal to Sacsin

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details

  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse

  • Immunogen

    Synthetic peptide corresponding to Human Sacsin aa 100-200 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab105487)

  • Positive control

    • This antibody gave a positive signal in human hippocampus formalin fixed paraffin embedded tissue section. ICC/IF: B35 and PC12 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab80195 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Co-chaperone which acts as a regulator of the Hsp70 chaperone machinery and may be involved in the processing of other ataxia-linked proteins.

  • Tissue specificity

    Highly expressed in the central nervous system. Also found in skeletal muscle and at low levels in pancreas.

  • Involvement in disease

    Spastic ataxia Charlevoix-Saguenay type

  • Sequence similarities

    Contains 1 HEPN domain.
    Contains 1 J domain.
    Contains 1 ubiquitin-like domain.

  • Domain

    The ubiquitin-like domain mediates interaction with the proteasome.
    The J domain is functional and is shown to stimulate E.coli dnaK ATPase activity.

  • Cellular localization

    Cytoplasm. Predominantly cytoplasmic, a small portion is present in the nucleus and also shows a partial mitochondrial overlap with the mitochondrial marker Hsp60.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • DnaJ homolog subfamily C member 29 antibody
    • DNAJC29 antibody
    • SACS antibody
    • SACS_HUMAN antibody
    • Sacsin antibody
    • Spastic ataxia of Charlevoix-Saguenay antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Sacsin antibody (ab80195)
    Immunocytochemistry/ Immunofluorescence - Anti-Sacsin antibody (ab80195)

    ab80195 staining Sacsin in B35 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with ab80195 (5µg/ml) overnight at +4°C (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). The secondary antibody (red) was Alexa Fluor® 594 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Nuclear DNA was labelled with DAPI (shown in blue).

    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sacsin antibody (ab80195)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sacsin antibody (ab80195)
    IHC image of Sacsin staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab80195, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Immunocytochemistry/ Immunofluorescence - Anti-Sacsin antibody (ab80195)
    Immunocytochemistry/ Immunofluorescence - Anti-Sacsin antibody (ab80195)
    IF image of ab80195 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80195, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

References (0)

Publishing research using ab80195? Please let us know so that we can cite the reference in this datasheet.

ab80195 has not yet been referenced specifically in any publications.

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Sacsin antibody (ab80195)
    Immunocytochemistry/ Immunofluorescence - Anti-Sacsin antibody (ab80195)

    ab80195 staining Sacsin in B35 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with ab80195 (5µg/ml) overnight at +4°C (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). The secondary antibody (red) was Alexa Fluor® 594 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Nuclear DNA was labelled with DAPI (shown in blue).

    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sacsin antibody (ab80195)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sacsin antibody (ab80195)
    IHC image of Sacsin staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab80195, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Immunocytochemistry/ Immunofluorescence - Anti-Sacsin antibody (ab80195)
    Immunocytochemistry/ Immunofluorescence - Anti-Sacsin antibody (ab80195)
    IF image of ab80195 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80195, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

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