Human Podocin ELISA Kit (ab245708)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1 pg/ml
- Range: 15.63 pg/ml - 1000 pg/ml
- Sample type: Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Podocin ELISA Kit
See all NPHS2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% extract 24 3.2% Inter-assay Sample n Mean SD CV% extract 3 3.7% -
Sample type
Tissue Extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
1 pg/ml -
Range
15.63 pg/ml - 1000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Tissue Extracts 98 87% - 103% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Podocin ELISA Kit (ab245708) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Podocin protein in tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Podocin with 1 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Podocin Capture Antibody 1 x 600µl 10X Human Podocin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BI 1 x 6ml Human Podocin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton. -
Tissue specificity
Almost exclusively expressed in the podocytes of fetal and mature kidney glomeruli. -
Involvement in disease
Defects in NPHS2 are the cause of nephrotic syndrome type 2 (NPHS2) [MIM:600995]. It is a renal disorder characterized clinically by childhood onset of proteinuria, hypoalbuminemia, hyperlipidemia, and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. The disorder is resistant to steroid treatment and progresses to end-stage renal failure in the first or second decades. Some patients show later onset of the disorder. -
Sequence similarities
Belongs to the band 7/mec-2 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Alternative names
- nephrosis 2
- nephrosis 2, idiopathic, steroid resistant
- Nephrosis 2, idiopathic, steroid resistant (podocin)
see all -
Database links
- Entrez Gene: 7827 Human
- Omim: 604766 Human
- SwissProt: Q9NP85 Human
- Unigene: 412710 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human Podocin standard curve in 1X Cell Extraction Buffer PTR.The Podocin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed. -
Interpolated concentrations of native Podocin in human fetal kidney tissue extract based on a 325 µg/mL extract load.The concentrations of Podocin were measured in duplicate and interpolated from the Podocin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Podocin concentration was determined to be 1102.2 pg/mL. -
Sandwich ELISA - Human Podocin ELISA Kit (ab245708)To learn more about the advantages of recombinant antibodies see here.
