Anti-ACAA1/Beta-ketothiolase antibody (ab241072)
Key features and details
- Rabbit polyclonal to ACAA1/Beta-ketothiolase
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ACAA1/Beta-ketothiolase antibody
See all ACAA1/Beta-ketothiolase primary antibodies -
Description
Rabbit polyclonal to ACAA1/Beta-ketothiolase -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human ACAA1/Beta-ketothiolase aa 50-100. The exact sequence is proprietary. (NP_001598.1).
Database link: P09110 -
Positive control
- WB: HeLa, HEK-293T and Jurkat cell lysate. IP: ACAA1/Beta-ketothiolase IP in Jurkat cell lysate.
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General notes
This product was previously labelled as ACAA1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ACAA1/Beta-ketothiolase antibody (ab241072)All lanes : Anti-ACAA1/Beta-ketothiolase antibody (ab241072) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Exposure time: 3 minutes
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Immunoprecipitation - Anti-ACAA1/Beta-ketothiolase antibody (ab241072)ACAA1/Beta-ketothiolase was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241072 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab241072 at 1 µg/ml.
Lane 1: ab241072 IP in Jurkat whole cell lysate.
Lane 2: Control IgG IP in Jurkat whole cell lysate.Detection: Chemiluminescence with exposure time of 30 seconds.
