Anti-ACAA1/Beta-ketothiolase antibody (ab228747)
Key features and details
- Rabbit polyclonal to ACAA1/Beta-ketothiolase
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ACAA1/Beta-ketothiolase antibody
See all ACAA1/Beta-ketothiolase primary antibodies -
Description
Rabbit polyclonal to ACAA1/Beta-ketothiolase -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow
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Immunogen
Recombinant fragment within Human ACAA1/Beta-ketothiolase (internal sequence). The exact sequence is proprietary.
Database link: P09110 -
Positive control
- WB: HepG2 whole cell lysate. ICC/IF: HeLa and HepG2 cells.
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General notes
This product was previously labelled as ACAA1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAA1/Beta-ketothiolase antibody (ab228747)
Paraffin-embeded mouse liver tissue labeling ACAA1/Beta-ketothiolase using ab228747 at 1/500 dilution in immunohistochemical analysis.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 minutes.
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Immunocytochemistry/ Immunofluorescence - Anti-ACAA1/Beta-ketothiolase antibody (ab228747)Ice-cold methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ACAA1/Beta-ketothiolase (green) using ab228747 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
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Western blot - Anti-ACAA1/Beta-ketothiolase antibody (ab228747)Anti-ACAA1/Beta-ketothiolase antibody (ab228747) at 1/1000 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
Predicted band size: 44 kDa10% SDS-PAGE gel.
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Immunocytochemistry/ Immunofluorescence - Anti-ACAA1/Beta-ketothiolase antibody (ab228747)Methanol-fixed HepG2 (human liver hepatocellular carcinoma cell line) cells stained for ACAA1/Beta-ketothiolase (green) using ab228747 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
