Anti-ACAA1/Beta-ketothiolase antibody (ab224235)
Key features and details
- Rabbit polyclonal to ACAA1/Beta-ketothiolase
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-ACAA1/Beta-ketothiolase antibody
See all ACAA1/Beta-ketothiolase primary antibodies -
Description
Rabbit polyclonal to ACAA1/Beta-ketothiolase -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human ACAA1/Beta-ketothiolase aa 50-200.
Database link: P09110 -
Positive control
- WB: RT4 and U-251 cell lysates; plasma, liver and tonsil tissue lysates IHC-P: Human colon, kidney, liver and pancreas tissue. ICC/IF: U-251 MG cells.
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General notes
This product was previously labelled as ACAA1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ACAA1/Beta-ketothiolase antibody (ab224235)All lanes : Anti-ACAA1/Beta-ketothiolase antibody (ab224235) at 0.4 µg/ml
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human plasma
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
Predicted band size: 44 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAA1/Beta-ketothiolase antibody (ab224235)Immunohistochemical analysis of human pancreas labeling ACAA1/Beta-ketothiolase with ab224235 at 1/50. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAA1/Beta-ketothiolase antibody (ab224235)Immunohistochemical analysis of human liver labeling ACAA1/Beta-ketothiolase with ab224235 at 1/50.
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Western blot - Anti-ACAA1/Beta-ketothiolase antibody (ab224235)All lanes : Anti-ACAA1/Beta-ketothiolase antibody (ab224235) at 1/100 dilution
Lane 1 : NIH/3T3 (mouse embyro fibroblast cell line) cell lysate
Lane 2 : NBT-II (rat Wistar bladder tumor cell line) cell lysate
Predicted band size: 44 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAA1/Beta-ketothiolase antibody (ab224235)Immunohistochemical analysis of human kidney labeling ACAA1/Beta-ketothiolase with ab224235 at 1/50.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAA1/Beta-ketothiolase antibody (ab224235)Immunohistochemical analysis of human colon labeling ACAA1/Beta-ketothiolase with ab224235 at 1/50.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAA1/Beta-ketothiolase antibody (ab224235)Immunohistocehmical analysis of paraffin-embedded human cerebral cortex tissue staining ACAA1 with ab224235 at 1/50 dilution.
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Immunocytochemistry/ Immunofluorescence - Anti-ACAA1/Beta-ketothiolase antibody (ab224235)PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for ACAA1/Beta-ketothiolase (green) using ab224235 (4 µg/ml) in ICC/IF.
