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Anti-ACAA1/Beta-ketothiolase antibody [2H6BE7] (ab110289)

Key features and details

  • Mouse monoclonal [2H6BE7] to ACAA1/Beta-ketothiolase
  • Suitable for: ICC, Flow Cyt, IP, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-ACAA1/Beta-ketothiolase antibody [2H6BE7]
    See all ACAA1/Beta-ketothiolase primary antibodies

  • Description

    Mouse monoclonal [2H6BE7] to ACAA1/Beta-ketothiolase

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC
    Human
    IP
    Mouse
    WB
    Human
    See all applications and species data

  • Immunogen

    Tissue, cells or virus. This information is considered to be commercially sensitive.

  • Positive control

    • HepG2,HL-60 cells, human liver tissue, rat liver tissue and mouse liver tissue.

  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

     This product was previously labelled as ACAA1

     

Properties

Images

  • Western blot - Anti-ACAA1/Beta-ketothiolase antibody [2H6BE7] (ab110289)
    Western blot - Anti-ACAA1/Beta-ketothiolase antibody [2H6BE7] (ab110289)
    All lanes : Anti-ACAA1/Beta-ketothiolase antibody [2H6BE7] (ab110289) at 1 µg/ml

    Lane 1 : Human liver
    Lane 2 : HepG2
    Lane 3 : Rat liver
    Lane 4 : Mouse liver

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 44 kDa

  • Immunoprecipitation - Anti-ACAA1/Beta-ketothiolase antibody [2H6BE7] (ab110289)
    Immunoprecipitation - Anti-ACAA1/Beta-ketothiolase antibody [2H6BE7] (ab110289)

    ACAA1/Beta-ketothiolase was immunoprecipitated using 0.5mg Mouse Liver tissue lysate, 5µg of Mouse monoclonal to ACAA1/Beta-ketothiolase and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, Mouse Liver tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab110289.

    Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/20,000 dilution.

    Band: 44kDa; ACAA1/Beta-ketothiolase

  • Immunocytochemistry - Anti-ACAA1/Beta-ketothiolase antibody [2H6BE7] (ab110289)
    Immunocytochemistry - Anti-ACAA1/Beta-ketothiolase antibody [2H6BE7] (ab110289)

    ab110289, at 5 µg/mL, staining ACAA1/Beta-ketothiolase in Human HepG2 cells. DAPI was used to stain the cell nuclei (blue).

  • Flow Cytometry - Anti-ACAA1/Beta-ketothiolase antibody [2H6BE7] (ab110289)
    Flow Cytometry - Anti-ACAA1/Beta-ketothiolase antibody [2H6BE7] (ab110289)

    ab110289, at 1 µg/mL, staining ACAA1/Beta-ketothiolase in HL-60 cells (blue) or using an isotype control (red).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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