Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-STK33 antibody [EPR15343] (ab206296)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15343] to STK33
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-STK33 antibody [EPR15343]
    See all STK33 primary antibodies

  • Description

    Rabbit monoclonal [EPR15343] to STK33

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB; Jurkat and HEK293 whole cell lysates, Human testis lysate, HUman fetal kidney and spleen lysates. IHC-P; Human testis and Human fetal cardiac muscle tissue. ICC/IF; Jurkat cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR15343

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-STK33 antibody [EPR15343] (ab206296)
    Western blot - Anti-STK33 antibody [EPR15343] (ab206296)
    All lanes : Anti-STK33 antibody [EPR15343] (ab206296) at 1/1000 dilution

    Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 2 : HEK293 (Human epithelial cell line from embryonic kidney) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 58, 50 kDa
    Observed band size: 58 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/dilution buffer: 5% NFDM/TBST.

     

    Can recognize two isoforms. The predicted MW are 58KDa and 50KDa, respectively

     

  • Western blot - Anti-STK33 antibody [EPR15343] (ab206296)
    Western blot - Anti-STK33 antibody [EPR15343] (ab206296)
    Anti-STK33 antibody [EPR15343] (ab206296) at 1/1000 dilution + Human testis lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/100000 dilution

    Predicted band size: 58, 50 kDa
    Observed band size: 58, 50 kDa


    Exposure time: 1 minute


    Blocking/dilution buffer: 5% NFDM/TBST.

     

    Can recognize two isoforms. The predicted MW are 58KDa and 50KDa, respectively

  • Western blot - Anti-STK33 antibody [EPR15343] (ab206296)
    Western blot - Anti-STK33 antibody [EPR15343] (ab206296)
    All lanes : Anti-STK33 antibody [EPR15343] (ab206296) at 1/1000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : Human fetal spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/100000 dilution

    Predicted band size: 58, 50 kDa
    Observed band size: 58 kDa why is the actual band size different from the predicted?



    Blocking/dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 10 seconds;
    Lane 2: 3 minutes

     

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STK33 antibody [EPR15343] (ab206296)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STK33 antibody [EPR15343] (ab206296)

    Immunohistochemical analysis of paraffin-embedded human testis tissue labeling STK33 with ab206296 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Nuclear and cytoplasmic staining on human testis tissue is observed.

    Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STK33 antibody [EPR15343] (ab206296)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STK33 antibody [EPR15343] (ab206296)

    Immunohistochemical analysis of paraffin-embedded human fetal cardiac tissue labeling STK33 with ab206296 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Nuclear and cytoplasmic staining on human fetal cardiac muscle tissue is observed.

    Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-STK33 antibody [EPR15343] (ab206296)
    Immunocytochemistry/ Immunofluorescence - Anti-STK33 antibody [EPR15343] (ab206296)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling STK33 with ab206296 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Cytoplasmic and nuclear staining on Jurkat cell line is observed.

    The nuclear counterstain is DAPI (blue).

    Tubulin is detected with anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594)  (ab150120) secondary antibody at 1/1000 dilution (red).
    The negative controls are as follows:-
    -ve control 1 - ab206296 at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution.
    -ve control 2. -  anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Anti-STK33 antibody [EPR15343] (ab206296)
    Anti-STK33 antibody [EPR15343] (ab206296)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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