Antibodies, Proteins, Kits and Reagents for Life Science

351 792 ₸ Product size

100 µl 351 792 ₸ 10 µl 117 264 ₸

Order now and get it on Friday July 23, 2021

Anti-SAA3 antibody [EPR23102-242] (ab233547)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR23102-242] to SAA3
Suitable for: Flow Cyt (Intra), WB, ICC, IP
Reacts with: Mouse, Rat

Product name

Anti-SAA3 antibody [EPR23102-242]
See all SAA3 primary antibodies
Description

Rabbit monoclonal [EPR23102-242] to SAA3
Host species

Rabbit
Tested applications

Suitable for: Flow Cyt (Intra), WB, ICC, IPmore details
Unsuitable for: IHC-Fr or IHC-P
Species reactivity

Reacts with: Mouse, Rat
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse colon, liver, RAW 264.7, J774A.1, NR8383, Rat colon, Rat liver, Rat spleen, His/lif-tagged mouse Saa3 recombinant protein lysates. ICC: RAW 264.7 cells. Flow Cyt (intra): RAW 264.7 cells. IP: Mouse colon tissue lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23102-242
Isotype

IgG

Western blot - Anti-SAA3 antibody [EPR23102-242] (ab233547)

All lanes : Anti-SAA3 antibody [EPR23102-242] (ab233547) at 1/1000 dilution

Lane 1 : Mouse colon tissue lysate
Lane 2 : Mouse liver tissue lysate
Lane 3 : Mouse kidney tissue lysate
Lane 4 : Mouse heart tissue lysate
Lane 5 : RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 6 : J774A.1 (mouse reticulum cell sarcoma monocyte macrophage) whole cell lysate
Lane 7 : NR8383 (rat norvegicus lung macrophage (alveolar)) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Lanes 1 & 3-7 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Lane 2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Observed band size: 14 kDa
why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST

Low expression: kidney and heart (PMID:29056547)

Exposure time: 92 seconds
Immunocytochemistry - Anti-SAA3 antibody [EPR23102-242] (ab233547)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling SAA3 with ab233547 at 1/50 dilution (9.18 ug/ml), followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in RAW 264.7 cells is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Flow Cytometry - Anti-SAA3 antibody [EPR23102-242] (ab233547)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling SAA3 with ab233547 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-SAA3 antibody [EPR23102-242] (ab233547)

SAA3 was immunoprecipitated from 0.35 mg of Mouse colon tissue lysate with ab233547 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab233547 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: Mouse colon tissue lysate 10 ug

Lane 2: ab233547 IP in Mouse colon tissue lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab233547 in Mouse colon tissue lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 15 seconds
Western blot - Anti-SAA3 antibody [EPR23102-242] (ab233547)

All lanes : Anti-SAA3 antibody [EPR23102-242] (ab233547) at 1/1000 dilution

Lane 1 : Rat colon tissue lysate
Lane 2 : Rat liver tissue lysate
Lane 3 : Rat spleen tissue lysate
Lane 4 : Rat kidney tissue lysate
Lane 5 : Rat heart tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Observed band size: 14 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST

Low expression: kidney and heart (PMID:29056547)

Exposure time: 92 seconds
Western blot - Anti-SAA3 antibody [EPR23102-242] (ab233547)

All lanes : Anti-SAA3 antibody [EPR23102-242] (ab233547) at 1/1000 dilution

Lane 1 : His-tagged mouse Saa1 recombinant protein (aa 20-122) at 0.02 µg
Lane 2 : E.coli extracts containing his-tagged mouse Saa2 recombinant protein (aa 20-122) at 20 µl
Lane 3 : His/lif-tagged mouse Saa3 recombinant protein (aa 19-122) at 0.01 µg

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Blocking and diluting buffer and concentration: 5% NFDM/TBST

Exposure time: 26 seconds
Anti-SAA3 antibody [EPR23102-242] (ab233547)

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