Human CA9 ELISA Kit (ab267572)
Key features and details
- Sensitivity: 0.2 pg/ml
- Range: 0.2 pg/ml - 50 pg/ml
- Sample type: Cell culture media, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (qualitative)
- Reacts with: Human
Overview
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Product name
Human CA9 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Serum, Plasma, Cell culture media -
Assay type
Sandwich (qualitative) -
Sensitivity
0.2 pg/ml -
Range
0.2 pg/ml - 50 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 124 110% - 137% Plasma 116 100% - 141% Cell culture media 117 101% - 131% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human CA9 ELISA Kit is designed for the quantitative determination of CA9 in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for human CA9 coated on a 96-well plate. Standards and samples are pipetted into the wells and CA9 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human CA9 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CA9 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA- 78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, FGF-4, FGF-6, FGF- 7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNFbeta, TPO, VEGF.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Anti-Human CA9 coated Microplate (12 x 8 wells) 1 unit Assay Diluent C 1 x 30ml Biotinylated Anti-Human CA9 Detection Antibody 2 vials Human CA9 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia. -
Tissue specificity
Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa. -
Sequence similarities
Belongs to the alpha-carbonic anhydrase family.
Contains 1 alpha-carbonic anhydrase domain. -
Post-translational
modificationsAsn-346 bears high-mannose type glycan structures. -
Cellular localization
Nucleus. Nucleus, nucleolus. Cell membrane. Cell projection, microvillus membrane. Found on the surface microvilli and in the nucleus, particularly in nucleolus. - Information by UniProt
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Alternative names
- CA-IX
- CA9
- CAH9_HUMAN
see all -
Database links
- Entrez Gene: 768 Human
- Omim: 603179 Human
- SwissProt: Q16790 Human
- Unigene: 63287 Human
Images
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Standard curve
Human CA9 ELISA kit (ab267572) Standard curve.
Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.