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Signal Transduction Growth Factors/Hormones Hormones

Anti-CGA/hCG alpha antibody [EP3373] (ab92738)

Price and availability

291 484 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-CGA/hCG alpha antibody [EP3373] (ab92738)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit monoclonal [EP3373] to CGA/hCG alpha
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-CGA/hCG alpha antibody [EP3373]
    See all CGA/hCG alpha primary antibodies
  • Description

    Rabbit monoclonal [EP3373] to CGA/hCG alpha
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CGA/hCG alpha aa 1-100. The exact sequence is proprietary.
    Database link: P01215

  • Positive control

    • WB: JAR cell lysates treated with hydroxyurea. Human placenta lysate. IHC-P: Human placenta tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP3373
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Growth Factors/Hormones
    • Hormones
    • Neuroscience
    • Endocrine system
    • Thyroid axis
    • Neuroscience
    • Endocrine system
    • Gonadotrophic axis
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Hormone biosynthesis
    • Metabolism
    • Pathways and Processes
    • Endocrine metabolism
    • Hormone biosynthesis
    • Metabolism
    • Types of disease
    • Cancer

Images

  • Western blot - Anti-CGA/hCG alpha antibody [EP3373] (ab92738)
    Western blot - Anti-CGA/hCG alpha antibody [EP3373] (ab92738)
    Anti-CGA/hCG alpha antibody [EP3373] (ab92738) at 1/5000 dilution (purified) + human placenta at 10 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 13 kDa
    Observed band size: 18 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-CGA/hCG alpha antibody [EP3373] (ab92738)
    Western blot - Anti-CGA/hCG alpha antibody [EP3373] (ab92738)
    Anti-CGA/hCG alpha antibody [EP3373] (ab92738) at 1/1000 dilution (purified) + Hydroxyurea treated JAR (Human placenta choriocarcinoma cell line) whole cell lysate at 10 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 13 kDa
    Observed band size: 18 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGA/hCG alpha antibody [EP3373] (ab92738)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGA/hCG alpha antibody [EP3373] (ab92738)

    Immunohistochemical staining of paraffin embedded human placenta with purified ab92738 at a working dilution of 1/2000. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGA/hCG alpha antibody [EP3373] (ab92738)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGA/hCG alpha antibody [EP3373] (ab92738)
    Immunohistochemical staining of paraffin embedded human pancreas with purified ab92738 at a working dilution of 1/2000. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Western blot - Anti-CGA/hCG alpha antibody [EP3373] (ab92738)
    Western blot - Anti-CGA/hCG alpha antibody [EP3373] (ab92738)
    All lanes : Anti-CGA/hCG alpha antibody [EP3373] (ab92738) at 1/500 dilution (unpurified)

    Lane 1 : JAR (Human placenta choriocarcinoma cell line) cell lysates, treated with
    hydroxyurea
    Lane 2 : JAR cell lysates, untreated

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 13 kDa
    Observed band size: 18 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGA/hCG alpha antibody [EP3373] (ab92738)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGA/hCG alpha antibody [EP3373] (ab92738)

    Unpurified ab92738 showing negative staining in normal breast tissue.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGA/hCG alpha antibody [EP3373] (ab92738)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGA/hCG alpha antibody [EP3373] (ab92738)

    Unpurified ab92738 showing negative staining in normal kidney tissue.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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