Anti-CGA/hCG alpha antibody [EP3373] (ab92738)
Key features and details
- Rabbit monoclonal [EP3373] to CGA/hCG alpha
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CGA/hCG alpha antibody [EP3373]
See all CGA/hCG alpha primary antibodies -
Description
Rabbit monoclonal [EP3373] to CGA/hCG alpha -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human CGA/hCG alpha aa 1-100. The exact sequence is proprietary.
Database link: P01215 -
Positive control
- WB: JAR cell lysates treated with hydroxyurea. Human placenta lysate. IHC-P: Human placenta tissue.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP3373 -
Isotype
IgG -
Research areas
Images
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Anti-CGA/hCG alpha antibody [EP3373] (ab92738) at 1/5000 dilution (purified) + human placenta at 10 µg
Secondary
HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 13 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
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Anti-CGA/hCG alpha antibody [EP3373] (ab92738) at 1/1000 dilution (purified) + Hydroxyurea treated JAR (Human placenta choriocarcinoma cell line) whole cell lysate at 10 µg
Secondary
HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 13 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
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Immunohistochemical staining of paraffin embedded human placenta with purified ab92738 at a working dilution of 1/2000. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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Immunohistochemical staining of paraffin embedded human pancreas with purified ab92738 at a working dilution of 1/2000. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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All lanes : Anti-CGA/hCG alpha antibody [EP3373] (ab92738) at 1/500 dilution (unpurified)
Lane 1 : JAR (Human placenta choriocarcinoma cell line) cell lysates, treated with
hydroxyurea
Lane 2 : JAR cell lysates, untreated
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 13 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
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Unpurified ab92738 showing negative staining in normal breast tissue.
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Unpurified ab92738 showing negative staining in normal kidney tissue.