Anti-14-3-3 zeta antibody (ab51129)
Key features and details
- Rabbit polyclonal to 14-3-3 zeta
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-14-3-3 zeta antibody
See all 14-3-3 zeta primary antibodies -
Description
Rabbit polyclonal to 14-3-3 zeta -
Host species
Rabbit -
Specificity
ab51129 detects endogenous levels of total 14-3-3 zeta protein.
Cross reactivity of ab51129 antibody with other member of the 14-3-3 family:
-92% identities with 14-3-3 beta/alpha, gamma and eta
-77% identities with 14-3-3 teta
-69% identities with 14-3-3 epsilon
-62% identities with 14-3-3 sigma -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human 14-3-3 zeta. The immunogen is a 13 amino acid synthetic peptide that resides within amino acids 24-73 of the human 14-3-3 zeta.
Database link: P63104 -
Positive control
- WB: 293 cells IHC-P: Human glioblastoma, human breast carcinoma tissue. ICC/IF: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-14-3-3 zeta antibody (ab51129)All lanes : Anti-14-3-3 zeta antibody (ab51129)
Lane 1 : Extracts from 293 cells treated
with Forskolin (40nM, 30min) with no peptide
Lane 2 : Extracts from 293 cells treated
with Forskolin (40nM, 30min) with immunising peptide
Predicted band size: 28 kDa
Observed band size: 28 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 zeta antibody (ab51129) Simeone P et al., PLoS One 9:e103030 (2014), Fig 7., doi: 10.1371/journal.pone.0103030 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/ab51129 staining 14-3-3 zeta in Human glioblastoma samples (A) and low grade tumors (B) by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/50) overnight. Nuclei stained blue with hematoxylin. Scale bars = 20 µm
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Western blot - Anti-14-3-3 zeta antibody (ab51129) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 zeta antibody (ab51129)This image shows paraffin-embedded human breast carcinoma tissue stained with ab51129 at a dilution of 1/100. Right hand image: tissue treated with immunising peptide; left hand image: untreated tissue -
Immunocytochemistry/ Immunofluorescence - Anti-14-3-3 zeta antibody (ab51129)ICC/IF image of ab51129 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51129, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
