Anti-Tat-SF1 antibody [EPR9105(B)] (ab134921)
![Anti-Tat-SF1 antibody [EPR9105(B)] (ab134921)](https://www.abcam.com/ps/products/134/ab134921/Images/HIV-TAT-specific-factor-1-Primary-antibodies-ab134921-1.jpg "Anti-Tat-SF1 antibody [EPR9105(B)] (ab134921)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9105(B)] to Tat-SF1
- Suitable for: WB, IHC-P, ICC/IF, IP
- Reacts with: Rat, Human
Overview
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Product name
Anti-Tat-SF1 antibody [EPR9105(B)]
See all Tat-SF1 primary antibodies -
Description
Rabbit monoclonal [EPR9105(B)] to Tat-SF1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, IPmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide within Human Tat-SF1 aa 350-450. The exact sequence is proprietary.
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Positive control
- HeLa and Jurkat cell lysates. Human colon tissue. HepG2 cells.
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General notes
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Previously labelled as HIV TAT specific factor 1.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. -
Dissociation constant (KD)
KD = 8.00 x 10 -12 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9105(B) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Tat-SF1 antibody [EPR9105(B)] (ab134921)All lanes : Anti-Tat-SF1 antibody [EPR9105(B)] (ab134921) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 86 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tat-SF1 antibody [EPR9105(B)] (ab134921)](https://www.abcam.com/ps/products/134/ab134921/Images/HIV-TAT-specific-factor-1-Primary-antibodies-ab134921-2.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tat-SF1 antibody [EPR9105(B)] (ab134921)Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling Tat-SF1 with ab134921 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tat-SF1 antibody [EPR9105(B)] (ab134921)](https://www.abcam.com/ps/products/134/ab134921/Images/HIV-TAT-specific-factor-1-Primary-antibodies-ab134921-3.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tat-SF1 antibody [EPR9105(B)] (ab134921)Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling Tat-SF1 with ab134921 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-Tat-SF1 antibody [EPR9105(B)] (ab134921)](https://www.abcam.com/ps/products/134/ab134921/Images/HIV-TAT-specific-factor-1-Primary-antibodies-ab134921-4.JPG)
Immunocytochemistry/ Immunofluorescence - Anti-Tat-SF1 antibody [EPR9105(B)] (ab134921)Immunofluorescence analysis of HepG2 cells staining Tat-SF1 with ab134921 at 1/250 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tat-SF1 antibody [EPR9105(B)] (ab134921)](https://www.abcam.com/ps/products/134/ab134921/Images/HIV-TAT-specific-factor-1-Primary-antibodies-ab134921-5.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tat-SF1 antibody [EPR9105(B)] (ab134921)Immunohistochemical analysis using ab134921 showing positive staining in Normal testis tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tat-SF1 antibody [EPR9105(B)] (ab134921)](https://www.abcam.com/ps/products/134/ab134921/Images/HIV-TAT-specific-factor-1-Primary-antibodies-ab134921-6.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tat-SF1 antibody [EPR9105(B)] (ab134921)Immunohistochemical analysis using ab134921 showing positive staining in Breast carcinoma tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-Tat-SF1 antibody [EPR9105(B)] (ab134921)](https://www.abcam.com/ps/products/134/ab134921/Images/HIV-TAT-specific-factor-1-Primary-antibodies-ab134921-7.jpg)
OI-RD Scanning - Anti-Tat-SF1 antibody [EPR9105(B)] (ab134921) -
![Anti-Tat-SF1 antibody [EPR9105(B)] (ab134921)](https://www.abcam.com/ps/products/134/ab134921/Images/ab134921-8-benefits-of-recombinant-antibodies.png)
Anti-Tat-SF1 antibody [EPR9105(B)] (ab134921)
