Anti-Tat-SF1 antibody [EPR9104(B)] (ab140654)
![Anti-Tat-SF1 antibody [EPR9104(B)] (ab140654)](https://www.abcam.com/ps/products/140/ab140654/Images/HIV-TAT-specific-factor-1-Primary-antibodies-ab140654-1.JPG "Anti-Tat-SF1 antibody [EPR9104(B)] (ab140654)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9104(B)] to Tat-SF1
- Suitable for: WB, IHC-P, IP, ICC/IF
- Reacts with: Mouse, Human
Overview
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Product name
Anti-Tat-SF1 antibody [EPR9104(B)]
See all Tat-SF1 primary antibodies -
Description
Rabbit monoclonal [EPR9104(B)] to Tat-SF1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IP, ICC/IFmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human Tat-SF1 aa 1-100. The exact sequence is proprietary.
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Positive control
- Jurkat and HeLa cell lysates; Human colon and uterus tissue; HeLa cells.
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General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Previously labelled as HIV TAT specific factor 1.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9104(B) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Tat-SF1 antibody [EPR9104(B)] (ab140654)All lanes : Anti-Tat-SF1 antibody [EPR9104(B)] (ab140654) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 86 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tat-SF1 antibody [EPR9104(B)] (ab140654)](https://www.abcam.com/ps/products/140/ab140654/Images/HIV-TAT-specific-factor-1-Primary-antibodies-ab140654-2.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tat-SF1 antibody [EPR9104(B)] (ab140654)Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling Tat-SF1 with ab140654 at 1/250 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tat-SF1 antibody [EPR9104(B)] (ab140654)](https://www.abcam.com/ps/products/140/ab140654/Images/HIV-TAT-specific-factor-1-Primary-antibodies-ab140654-3.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tat-SF1 antibody [EPR9104(B)] (ab140654)Immunohistochemical analysis of paraffin-embedded Human uterus tissue labelling Tat-SF1 with ab140654 at 1/250 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-Tat-SF1 antibody [EPR9104(B)] (ab140654)](https://www.abcam.com/ps/products/140/ab140654/Images/HIV-TAT-specific-factor-1-Primary-antibodies-ab140654-4.JPG)
Immunocytochemistry/ Immunofluorescence - Anti-Tat-SF1 antibody [EPR9104(B)] (ab140654)Immunofluorescent analysis of HeLa cells labelling Tat-SF1 with ab140654 at 1/250 dilution.
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![Anti-Tat-SF1 antibody [EPR9104(B)] (ab140654)](https://www.abcam.com/ps/products/140/ab140654/Images/ab140654-5-benefits-of-recombinant-antibodies.png)
Anti-Tat-SF1 antibody [EPR9104(B)] (ab140654)
