Anti-Cyclin T1 antibody (ab264325)
Key features and details
- Rabbit polyclonal to Cyclin T1
- Suitable for: IP, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Cyclin T1 antibody
See all Cyclin T1 primary antibodies -
Description
Rabbit polyclonal to Cyclin T1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee
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Immunogen
Synthetic peptide within Human Cyclin T1 aa 325-375. The exact sequence is proprietary. NP_001231.2
Database link: O60563 -
Positive control
- IHC-P: Human breast carcinoma tissue. WB: HeLa and HEK-293T whole cell lysate. IP: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Cyclin T1 antibody (ab264325)All lanes : Anti-Cyclin T1 antibody (ab264325) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HEK-293T whole cell lysate at 50 µg
Predicted band size: 81 kDa
Exposure time: 30 seconds4-8% SDS-PAGE.
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Immunoprecipitation - Anti-Cyclin T1 antibody (ab264325)Cyclin T1 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264325 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab264325 at 1 µg/ml.
Lane 1: ab264325 IP in HeLa whole cell lysate.
Lane 2: Control IgG.Exposure time: 3 secs.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin T1 antibody (ab264325)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast carcinoma tissue labeling Cyclin T1 with ab264325 at 1/200 dilution. Detection: DAB.
