Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cyclin T1 antibody [EPR17982] - BSA and Azide free (ab238940)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17982] to Cyclin T1 - BSA and Azide free
  • Suitable for: Flow Cyt, WB, IP, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Cyclin T1 antibody [EPR17982] - BSA and Azide free
    See all Cyclin T1 primary antibodies

  • Description

    Rabbit monoclonal [EPR17982] to Cyclin T1 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    Note that the antibody detects the target protein from human cell lysates but not tissue lysates.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    ICC/IF
    Human
    IHC-P
    Mouse
    IP
    Rat
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Rat kidney tissue.

  • General notes

    Ab238940 is the carrier-free version of ab184703. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab238940 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Cyclin T1 antibody [EPR17982] - BSA and Azide free (ab238940)
    Immunocytochemistry/ Immunofluorescence - Anti-Cyclin T1 antibody [EPR17982] - BSA and Azide free (ab238940)

    Ab184703 staining Cyclin T1 in MCF7 (human breast adenocarcinoma epithelial cell). Cells were fixed with 100% Methanol. Samples were incubated with primary antibody at 1:100 dilution (6.4 μg/ml). An Alexa Fluor® 488 Goat anti-rabbit (ab150077) was used as the secondary antibody at 1/1000 dilution (2 μg/ml). Ab195888, anti-alpha Tubulin [DM1A] – Microtubule Marker (Alexa Fluor® 594) was used as counterstain antibody at 1/200 dilution (2.5 μg/ml). DAPI was used as a nuclear counterstain. Confocal image showing nuclear staining in MCF7 cell line.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184703).

  • Immunocytochemistry/ Immunofluorescence - Anti-Cyclin T1 antibody [EPR17982] - BSA and Azide free (ab238940)
    Immunocytochemistry/ Immunofluorescence - Anti-Cyclin T1 antibody [EPR17982] - BSA and Azide free (ab238940)

    Ab184703 staining Cyclin T1 in Jurkat (human T cell leukemia T lymphocyte). Cells were fixed with 100% Methanol. Samples were incubated with primary antibody at 1/100 dilution (6.4µg/ml). An Alexa Fluor® 488 Goat anti-rabbit (ab150077) was used as the secondary antibody at 1/1000 dilution (2 μg/ml). Ab195888, anti-alpha Tubulin [DM1A] – Microtubule Marker (Alexa Fluor® 594) was used as counterstain antibody at 1/200 dilution (2.5 μg/ml). DAPI was used as a nuclear counterstain. Confocal image showing nuclear staining in Jurkat cell line.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184703).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin T1 antibody [EPR17982] - BSA and Azide free (ab238940)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin T1 antibody [EPR17982] - BSA and Azide free (ab238940)

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Cyclin T1 with ab184703 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on hepatocytes of mouse liver is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184703).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-Cyclin T1 antibody [EPR17982] - BSA and Azide free (ab238940)
    Flow Cytometry - Anti-Cyclin T1 antibody [EPR17982] - BSA and Azide free (ab238940)

    Flow cytometric analysis of 4% paraformaldehyde-fixed NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling Cyclin T1 with ab184703 at 1/120 dilution (red) compared with a Rabbit IgG,monoclonal [EPR17982] -  Isotype Control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184703).

  • Immunoprecipitation - Anti-Cyclin T1 antibody [EPR17982] - BSA and Azide free (ab238940)
    Immunoprecipitation - Anti-Cyclin T1 antibody [EPR17982] - BSA and Azide free (ab238940)

    Cyclin T1 was immunoprecipitated from 1mg of PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate with ab184703 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab184703 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
    Lane 1: PC-12 whole cell lysate 10µg (Input).
    Lane 2: ab184703 IP in PC-12 whole cell lysate.
    Lane 3: Rabbit IgG,monoclonal [EPR17982] - Isotpe Control (ab172730) instead of ab184703 in PC-12 whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 30 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184703).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin T1 antibody [EPR17982] - BSA and Azide free (ab238940)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin T1 antibody [EPR17982] - BSA and Azide free (ab238940)

    Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling Cyclin T1 with ab184703 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on rat kidney is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184703).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-Cyclin T1 antibody [EPR17982] - BSA and Azide free (ab238940)
    Anti-Cyclin T1 antibody [EPR17982] - BSA and Azide free (ab238940)

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