Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SIRT2 antibody [EPR20411-105] - BSA and Azide free (ab223534)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20411-105] to SIRT2 - BSA and Azide free
  • Suitable for: IHC-Fr, WB, IHC-P, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SIRT2 antibody [EPR20411-105] - BSA and Azide free
    See all SIRT2 primary antibodies

  • Description

    Rabbit monoclonal [EPR20411-105] to SIRT2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-Fr, WB, IHC-P, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal brain and brain cortex lysates; Mouse brain and cerebral cortex lysates; Rat brain lysate; MCF7 whole cell lysate. IHC-P: Human, mouse and rat cerebrum tissues. IHC-Fr: Mouse striatum tissue. IP: Human fetal brain lysate.

  • General notes

    Ab223534 is the carrier-free version of ab211033. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab223534 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRT2 antibody [EPR20411-105] - BSA and Azide free (ab223534)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRT2 antibody [EPR20411-105] - BSA and Azide free (ab223534)

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling SIRT2 with ab211033 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on myelinated axons of human cerebrum is observed [PMID: 17447894]. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211033).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRT2 antibody [EPR20411-105] - BSA and Azide free (ab223534)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRT2 antibody [EPR20411-105] - BSA and Azide free (ab223534)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling SIRT2 with ab211033 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on myelinated axons of mouse cerebrum is observed [PMID: 17447894]. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211033).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRT2 antibody [EPR20411-105] - BSA and Azide free (ab223534)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRT2 antibody [EPR20411-105] - BSA and Azide free (ab223534)

    Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling SIRT2 with ab211033 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on myelinated axons of rat cerebrum is observed [PMID: 17447894]. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211033).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-SIRT2 antibody [EPR20411-105] - BSA and Azide free (ab223534)
    Immunoprecipitation - Anti-SIRT2 antibody [EPR20411-105] - BSA and Azide free (ab223534)

    SIRT2 was immunoprecipitated from 0.35 mg of Human fetal brain lysate with ab211033 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab211033 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Human fetal brain lysate, 10 μg (Input).

    Lane 2: ab211033 IP in Human fetal brain lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab211033 in Human fetal brain lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211033).

  • Immunohistochemistry (Frozen sections) - Anti-SIRT2 antibody [EPR20411-105] - BSA and Azide free (ab223534)
    Immunohistochemistry (Frozen sections) - Anti-SIRT2 antibody [EPR20411-105] - BSA and Azide free (ab223534)

    This IHC data was generated using the same anti-SIRT2 antibody clone [EPR20411] in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (cat# ab211033).

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse striatum tissue labeling SIRT2 with ab211033 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasmic staining on mouse stratium is observed [PMID: 17447894]. The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

     

  • Anti-SIRT2 antibody [EPR20411-105] - BSA and Azide free (ab223534)
    Anti-SIRT2 antibody [EPR20411-105] - BSA and Azide free (ab223534)

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