Anti-SIRT2 antibody (ab67299)
Key features and details
- Rabbit polyclonal to SIRT2
- Suitable for: WB, ICC
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-SIRT2 antibody
See all SIRT2 primary antibodies -
Description
Rabbit polyclonal to SIRT2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICCmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cynomolgus monkey, Common marmoset, Orangutan
-
Immunogen
Synthetic peptide:
LEDLVRREHA NI
conjugated to KLH, corresponding to amino acids 341-352 of Mouse SIR2/ SIRT2 -
Positive control
- WB: Whole extracts of mouse and rat brain ICC: HeLa cells
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-SIRT2 antibody (ab67299)All lanes : Anti-SIRT2 antibody (ab67299) at 2 µg/ml
Lane 1 : Whole extracts of mouse brain
Lane 2 : Whole extracts of rat brain
Lane 3 : Whole extracts of mouse brain with SIRT2 immunizing peptide
Lane 4 : Whole extracts of rat brain with SIRT2 immunizing peptide
Secondary
All lanes : Goat Anti-Rabbit IgG-Peroxidase
Predicted band size: 43 kDachemiluminescent substrate.
-
Immunocytochemistry - Anti-SIRT2 antibody (ab67299)Immunocytochemistry analysis of HeLa cells labeling SIRT2 with ab67299 at 10 μg/mL. Cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate. -
Immunocytochemistry - Anti-SIRT2 antibody (ab67299) Image from Stettner M et al., PLoS One. 2013;8(6):e66079. Fig 5.; doi: 10.1371/journal.pone.0066079. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/ab67299 staining SIRT2 in pure rat Schwann cells by ICC/IF (Immunocytochemistry/immunofluorescence) after resveratol (RSV) treatment. Cells were fixed with 4% PFA blocked with 10% Goat serum/ 0.1% Triton x-100/ 0.1% BSA in PBS for 60 minutes at 21°C followed by 10% Goat serum/ 0.5% Triton X-100/ 0.01% BSA in PBS for 15 minutes at 21°C. Samples were incubated with primary antibody (1/100 in PBS + 10% goat serum) overnight at 21°C. An Alexa Fluor®488-conjugated goat anti-mouse IgG polyclonal (1/400) was used as the secondary antibody. Stimulation of rSCs with RSV led to an increase of SIRT2 expression in densitometry analysis.
