Human HDAC6 knockout HeLa cell pellet (ab278820)
Overview
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Product name
Human HDAC6 knockout HeLa cell pellet
See all HDAC6 kits -
Product overview
Abcam’s knockout cell pellets give you access to native proteins, without the need to culture cells. Our knockout cell pellets are prepared from our single-gene knockout cell lines and provide an additional offering to our cell lysates.
Cells are snap-frozen to provide high quality pellets that are suitable for extraction with alternative lysis buffers or for preparation of lysates from subcellular fractions. Our knockout cell pellets are suitable for a variety of applications, including PCR, gene expression profiling and DNA library preparation. -
Parental Cell Line
HeLa -
Organism
Human -
Mutation description
Knockout achieved by using CRISPR/Cas9, 3 bp deletion in exon 3 and 5 bp deletion in exon 3. -
Passage number
Knockout validation
Sanger Sequencing, Western Blot (WB)Notes
Pellet size: 5 million cells/vial.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Tested applications
Suitable for: WBmore detailsProperties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit Human HDAC6 knockout HeLa cell pellet 1 vial Human wild-type HeLa cell pellet 1 vial -
Research areas
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Cell type
epithelial -
Disease
Adenocarcinoma -
Gender
Female -
STR Analysis
Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8, 12 CSF1PO: 9, 10
Target
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Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin. -
Sequence similarities
Belongs to the histone deacetylase family. HD type 2 subfamily.
Contains 1 UBP-type zinc finger. -
Post-translational
modificationsUbiquitinated. Its polyubiquitination however does not lead to its degradation.
Sumoylated in vitro. -
Cellular localization
Nucleus. Cytoplasm. It is mainly cytoplasmic, where it is associated with microtubules. - Information by UniProt
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Alternative names
- CPBHM
- FLJ16239
- HD 6
see all
Properties
-
Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit Human HDAC6 knockout HeLa cell pellet 1 vial Human wild-type HeLa cell pellet 1 vial -
Research areas
-
Cell type
epithelial -
Disease
Adenocarcinoma -
Gender
Female -
STR Analysis
Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8, 12 CSF1PO: 9, 10
Images
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Western blot - Human HDAC6 knockout HeLa cell pellet (ab278820)Lane 1: Wild-type HeLa cell lysate (20µg)
Lane 2: HDAC6 knockout HeLa cell lysate (20µg)
Lanes 1- 2: Merged signal (red and green). Green - ab133493 observed at 160 kDa. Red - loading control ab8245 observed at 37 kDa.
ab133493 Anti-HDAC6 antibody [EPR1698(2)] was shown to specifically react with HDAC6 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab264804 (knockout cell lysate ab257145) was used. Wild-type and HDAC6 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab133493 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 10000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Sanger Sequencing - Human HDAC6 knockout HeLa cell pellet (ab278820)Allele-1: 5 bp deletion in exon 3; Allele-2: 3 bp deletion in exon 3