Anti-CREBBP antibody - ChIP Grade (ab10490)
Key features and details
- Rabbit polyclonal to CREBBP - ChIP Grade
- Suitable for: IP, ChIP, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-CREBBP antibody - ChIP Grade
See all CREBBP primary antibodies -
Description
Rabbit polyclonal to CREBBP - ChIP Grade -
Host species
Rabbit -
Tested applications
Suitable for: IP, ChIP, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
-
Immunogen
-
Positive control
- WB: HeLa whole cell lysate
-
General notes
Cyclic AMP-responsive enhancer binding protein (CREB) binding protein (CBP) and p300 are closely related transcriptional coactivators that have been shown to directly interact with many different DNA-binding transcription factors including nuclear hormone receptors, CREB (cyclic AMP-responsive enhancer binding protein), c-Fos, c-Jun/v-Jun, c-Myb/v-Myb, TFIIB and MyoD.Both CBP and p300 have been shown to display histone acetyltransferase (HAT) activity, capable of acetylating all four core histone particles in nucleosomes.As a result of HAT activity, it has been suggested CBP and p300 may play a direct role in activating chromatin for transcription.Single point mutations in CBP have been proposed as causative factors in the developmental abnormalities of Rubinstein-Taybi syndrome (RTS).Although both CBP and p300 appear to function similarly, the inability of p300 to rescue CBP malfunction iRTS suggests intrinsic functional differences between CBP and p300.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibodies were affinity purified using the peptide immobilized on solid support. -
Primary antibody notes
Cyclic AMP-responsive enhancer binding protein (CREB) binding protein (CBP) and p300 are closely related transcriptional coactivators that have been shown to directly interact with many different DNA-binding transcription factors including nuclear hormone receptors, CREB (cyclic AMP-responsive enhancer binding protein), c-Fos, c-Jun/v-Jun, c-Myb/v-Myb, TFIIB and MyoD.Both CBP and p300 have been shown to display histone acetyltransferase (HAT) activity, capable of acetylating all four core histone particles in nucleosomes.As a result of HAT activity, it has been suggested CBP and p300 may play a direct role in activating chromatin for transcription.Single point mutations in CBP have been proposed as causative factors in the developmental abnormalities of Rubinstein-Taybi syndrome (RTS).Although both CBP and p300 appear to function similarly, the inability of p300 to rescue CBP malfunction iRTS suggests intrinsic functional differences between CBP and p300. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
ChIP - Anti-CREBBP antibody - ChIP Grade (ab10490)
Sonicated chromatin prepared from U2OS cells was subjected to the ChIP procedure with ab10490 to CREBBP. Immunoprecipitated chromatin was analysed in the promoter region of c-FOS (active) and in exon 2 of MYO-D (inactive), values are % of inputs. 2–4 µg of ab10490 and 1-2x106 cells were used in each ChIP experiment.
Sergei Denissov, Prof. Henk Stunnenberg lab, University Nijmengen, NL
-
Western blot - Anti-CREBBP antibody - ChIP Grade (ab10490)All lanes : Anti-CREBBP antibody - ChIP Grade (ab10490) at 0.4 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Exposure time: 3 minutesDetected by chemiluminescence.
-
Immunoprecipitation - Anti-CREBBP antibody - ChIP Grade (ab10490)ab10490 immunoprecipitating CREBBP at 6 µg/ml lysate.
Lane 1: Anti-CREBBP antibody ab10489 in HeLa whole cell extract (1 mg per IP reaction; 20% of IP loaded).
Lane 2: Anti-CREBBP antibody ab10490 in HeLa whole cell extract (1 mg per IP reaction; 20% of IP loaded).
Lane 3: IgG control.
For blotting immunoprecipitated CREBBP, ab10490 was used at 1 µg/mL.
Detection: Chemiluminescence with an exposure time of 30 seconds.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREBBP antibody - ChIP Grade (ab10490)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling CREBBP with ab10490 at 1/500 (2µg/ml). Detection: DAB.
