Anti-PMM1 antibody [EPR17844] (ab202058)
![Anti-PMM1 antibody [EPR17844] (ab202058)](https://www.abcam.com/ps/products/202/ab202058/Images/ab202058-244522-ab202058-wb-1.jpg "Anti-PMM1 antibody [EPR17844] (ab202058)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17844] to PMM1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-PMM1 antibody [EPR17844]
See all PMM1 primary antibodies -
Description
Rabbit monoclonal [EPR17844] to PMM1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseRatHumanWB MouseRatHuman
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human fetal liver, fetal brain, fetal heart and fetal kidney lysates; HepG2, 293, C6, NIH/3T3 and PC-12 whole cell lysates; Mouse brain and rat brain lysates. IHC-P: Human liver, mouse cerebral cortex and rat pancreas tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17844 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PMM1 antibody [EPR17844] (ab202058)All lanes : Anti-PMM1 antibody [EPR17844] (ab202058) at 1/5000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
Lane 3 : 293 (Human epithelial cells from embryonic kidney) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 30 kDa
Observed band size: 30 kDa
Exposure time: 1 minuteBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-PMM1 antibody [EPR17844] (ab202058)](https://www.abcam.com/ps/products/202/ab202058/Images/ab202058-244521-ab202058-wb-2.jpg)
Western blot - Anti-PMM1 antibody [EPR17844] (ab202058)All lanes : Anti-PMM1 antibody [EPR17844] (ab202058) at 1/2000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal heart lysate
Lane 3 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 30 kDa
Observed band size: 30 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-PMM1 antibody [EPR17844] (ab202058)](https://www.abcam.com/ps/products/202/ab202058/Images/ab202058-244520-ab202058-wb-3.jpg)
Western blot - Anti-PMM1 antibody [EPR17844] (ab202058)All lanes : Anti-PMM1 antibody [EPR17844] (ab202058) at 1/2000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 30 kDa
Observed band size: 30 kDa
Exposure time: 30 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-PMM1 antibody [EPR17844] (ab202058)](https://www.abcam.com/ps/products/202/ab202058/Images/ab202058-244519-ab202058-wb-4.jpg)
Western blot - Anti-PMM1 antibody [EPR17844] (ab202058)All lanes : Anti-PMM1 antibody [EPR17844] (ab202058) at 1/5000 dilution
Lane 1 : C6 (Rat glial tumor cells) whole cell lysate
Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 3 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 30 kDa
Observed band size: 30 kDa
Exposure time: 30 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMM1 antibody [EPR17844] (ab202058)](https://www.abcam.com/ps/products/202/ab202058/Images/ab202058-244518-ab202058-ihc-1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMM1 antibody [EPR17844] (ab202058)Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling PMM1 with ab202058 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on Human liver tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMM1 antibody [EPR17844] (ab202058)](https://www.abcam.com/ps/products/202/ab202058/Images/ab202058-244517-ab202058-ihc-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMM1 antibody [EPR17844] (ab202058)Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling PMM1 with ab202058 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on mouse cerebral cortex is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMM1 antibody [EPR17844] (ab202058)](https://www.abcam.com/ps/products/202/ab202058/Images/ab202058-244516-ab202058-ihc-3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMM1 antibody [EPR17844] (ab202058)Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling PMM1 with ab202058 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on rat pancreas is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Anti-PMM1 antibody [EPR17844] (ab202058)](https://www.abcam.com/ps/products/202/ab202058/Images/ab202058-9-benefits-of-recombinant-antibodies.png)
Anti-PMM1 antibody [EPR17844] (ab202058)
