Glucose 6 Phosphate Dehydrogenase Assay Kit (Colorimetric) (ab102529)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 0.04 mU/well
Overview
-
Product name
Glucose 6 Phosphate Dehydrogenase Assay Kit (Colorimetric)
See all Glucose 6 Phosphate Dehydrogenase kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts -
Assay type
Enzyme activity -
Sensitivity
> 0.04 mU/well -
Assay time
1h 00m -
Product overview
Glucose 6 Phosphate Dehydrogenase Assay Kit (Colorimetric) (ab102529) is a simple, sensitive and rapid assay detects the activity of Glucose 6 Phosphate Dehydrogenase (G6PDH or G6PD) in a variety of samples.
In the G6PD assay protocol, glucose 6 phosphate is oxidized, which leads to the conversion of a nearly colorless probe to an intensely colored product with an absorbance at 450nm.
The G6PD assay kit can detect as low as 0.04mU G6PD per well.
G6PD assay protocol summary:
- add samples and positive control to wells
- add reaction mix
- analyze on a microplate reader for 5-30 min -
Platform
Microplate reader
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests G6PDH Assay Buffer WM 1 x 25ml G6PDH Developer Red 1 vial G6PDH Positive Control Green 1 vial G6PDH Substrate Orange 1 vial NADH Standard Yellow 1 vial -
Research areas
-
Relevance
Glucose 6 phosphate dehydrogenase (G6PDH) is a cytosolic enzyme in the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells (such as erythrocytes) by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). The NADPH in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. Of greater quantitative importance is the production of NADPH for tissues actively engaged in biosynthesis of fatty acids and/or isoprenoids, such as the liver, mammary glands, adipose tissue, and the adrenal glands. -
Alternative names
- EC=1.1.1.49
- G6PD
- G6PD1
see all
Images
-
Functional Studies - Glucose 6 Phosphate Dehydrogenase Colorimetric Assay Kit (ab102529)
G6PDH Activity measured in mouse tissue lysates. Protein concentration for samples varied from 7 mg/mL to 17 mg/mL. Samples were diluted 1-3 fold.
-
Functional Studies - Glucose 6 Phosphate Dehydrogenase Colorimetric Assay Kit (ab102529)G6PDH Activity measured in cell lysates.
Samples with the concentration of 4e6 cells/mL were used. Samples were undiluted.
-
Functional Studies - Glucose 6 Phosphate Dehydrogenase Colorimetric Assay Kit (ab102529)G6PDH Activity measured in biological fluids. Samples were undiluted.
-
Functional Studies - Glucose 6 Phosphate Dehydrogenase Colorimetric Assay Kit (ab102529)G6PDH Activity measured in mouse tissue lysates. Protein concentration for samples varied from 7 mg/mL to 17 mg/mL. Samples were undiluted.
-
Functional Studies - Glucose 6 Phosphate Dehydrogenase Colorimetric Assay Kit (ab102529)G6PDH Activity measured in cell lysates.
Samples with the concentration of 4e6 cells/mL were used. Samples were undiluted.
-
Functional Studies - Glucose 6 Phosphate Dehydrogenase Colorimetric Assay Kit (ab102529)G6PDH Activity measured in biological fluids. Samples undiluted.
-
Functional Studies - Glucose 6 Phosphate Dehydrogenase Colorimetric Assay Kit (ab102529)Example of positive control and pork liver samples test curves obtained using ab102529 -
Functional Studies - Glucose 6 Phosphate Dehydrogenase Assay Kit (ab102529)Example of Standard curve obtained using ab102529
