HRP Anti-Hexokinase II antibody [EPR20839] (ab237315)
![HRP Anti-Hexokinase II antibody [EPR20839] (ab237315)](https://www.abcam.com/ps/products/237/ab237315/Images/ab237315-1-IHCAP00419721.jpg "HRP Anti-Hexokinase II antibody [EPR20839] (ab237315)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR20839] to Hexokinase II
- Suitable for: IHC-P
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-Hexokinase II antibody [EPR20839]
See all Hexokinase II primary antibodies -
Description
HRP Rabbit monoclonal [EPR20839] to Hexokinase II -
Host species
Rabbit -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
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Immunogen
Recombinant fragment within Human Hexokinase II aa 1-500. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P52789 -
Positive control
- IHC-P: Human pancreas adenocarcinoma tissue sections.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20839 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Hexokinase II antibody [EPR20839] (ab237315)
IHC image of Hexokinase II staining in a section of formalin-fixed paraffin-embedded human pancreas adenocarcinoma*, performed on a Leica BOND™.
The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH9, epitope retrieval solution 2) for 20mins. The section was then incubated with abab237315, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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![HRP Anti-Hexokinase II antibody [EPR20839] (ab237315)](https://www.abcam.com/ps/products/237/ab237315/Images/ab237315-2-benefits-of-recombinant-antibodies.png)
HRP Anti-Hexokinase II antibody [EPR20839] (ab237315)
