Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HP1 alpha antibody [EPR5776] - BSA and Azide free (ab248560)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5776] to HP1 alpha - BSA and Azide free
  • Suitable for: Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-HP1 alpha antibody [EPR5776] - BSA and Azide free
    See all HP1 alpha primary antibodies

  • Description

    Rabbit monoclonal [EPR5776] to HP1 alpha - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    The immunogen used for this product shares 83% homology with CBX1/HP1 beta. Cross-reactivity with this protein has not been confirmed experimentally.

  • Tested applications

    Suitable for: Flow Cyt, WBmore details
    Unsuitable for: ICC/IF,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab248560 is the carrier-free version of ab133577. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab248560 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-HP1 alpha antibody [EPR5776] - BSA and Azide free (ab248560)
    Western blot - Anti-HP1 alpha antibody [EPR5776] - BSA and Azide free (ab248560)
    All lanes : Anti-HP1 alpha antibody [EPR5776] (ab133577) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : SH SY5Y cell lysate
    Lane 3 : MCF7 cell lysate
    Lane 4 : A431 cell lysate
    Lane 5 : C6 cell lysate
    Lane 6 : RAW 264.7 cell lysate
    Lane 7 : PC12 cell lysate
    Lane 8 : NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 22 kDa



    This data was developed using ab133577, the same antibody clone in a different buffer formulation.

  • Flow Cytometry - Anti-HP1 alpha antibody [EPR5776] - BSA and Azide free (ab248560)
    Flow Cytometry - Anti-HP1 alpha antibody [EPR5776] - BSA and Azide free (ab248560)
    This data was developed using ab133577, the same antibody clone in a different buffer formulation.Flow cytometrric analysis of HPA1 alpha in permeabilized SH SY5Y cells, using ab133577 at a 1/10 dilution (red) or a control rabbit IgG antibody (green).
  • Anti-HP1 alpha antibody [EPR5776] - BSA and Azide free (ab248560)
    Anti-HP1 alpha antibody [EPR5776] - BSA and Azide free (ab248560)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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