Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] - BSA and Azide free (ab172382)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [UDD2 10(12)] to LRRK2 (phospho S935) - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Human
Overview
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Product name
Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] - BSA and Azide free
See all LRRK2 primary antibodies -
Description
Rabbit monoclonal [UDD2 10(12)] to LRRK2 (phospho S935) - BSA and Azide free -
Host species
Rabbit -
Specificity
The antibody does not give a positive signal in U-87 MG, SH-SY-5Y and human fetal brain. Please contact our Scientific Support team if you have any question.
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Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: GFP LRRK2, GFP LRRK2 S910A, GFP LRRK2 S935A, LRRK2 WT MEF, LRRK2 WT MEF, Lymphoblastoid, NIH/3T3 and RAW 264.7 cell lysates.
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General notes
ab172382 is the carrier-free version of ab133450 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab172382 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This antibody was developed with support from The Michael J. Fox Foundation.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
UDD2 10(12) -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] - BSA and Azide free (ab172382) at 1/10000 dilution
Lane 1 : Wide type-LRRK2 overexpressed lysates
Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
Lane 3 : RAW 264.7(Mouse Abelson murine leukemia virus-induced tumor macrophage)whole cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 286 kDaExposure:
Lane 1 and 2: 5 seconds.
Lane 3: 180 seconds. -
All lanes : Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] (ab133450) at 1/5000 dilution
Lane 1 : WT-LRRK2 cell lysate - untreated
Lane 2 : WT-LRRK2 cell lysate - treated with Lambda phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 286 kDaBlocking/Dilution buffer and concentration: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133450).
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All lanes : Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] (ab133450) at 1/1000 dilution
Lane 1 : GFP LRRK2 lysate at 5 µg
Lane 2 : GFP LRRK2 S910A lysate at 5 µg
Lane 3 : GFP LRRK2 S935A lysate at 5 µg
Lane 4 : LRRK2 WT MEF lysate at 20 µg
Lane 5 : LRRK2 WT MEF lysate from LRRK2 IN1 treated cells at 20 µg
Lane 6 : LRRK2 KO MEF lysate at 20 µg
Lane 7 : LRRK2 KO MEF lysate from LRRK2 IN1 treated cells at 20 µg
Lane 8 : Lymphoblastoid lysate at 30 µg
Lane 9 : Lymphoblastoid lysate from LRRK2 IN1 treated cells at 30 µg
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 286 kDaThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133450).
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