Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] - BSA and Azide free (ab209422)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1536Y] to Alpha-synuclein (phospho S129) - BSA and Azide free
  • Suitable for: IHC-P, ELISA, WB, Dot blot
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] - BSA and Azide free
    See all Alpha-synuclein primary antibodies

  • Description

    Rabbit monoclonal [EP1536Y] to Alpha-synuclein (phospho S129) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ELISA, WB, Dot blotmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab188826)

  • Positive control

    • Fetal brain lysate.

  • General notes

    ab209422 is the carrier-free version of ab51253 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab209422 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] - BSA and Azide free (ab209422)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] - BSA and Azide free (ab209422)

    This IHC data was generated using the same anti-phospho alpha synuclein S129 antibody clone, EP1536Y, in a different buffer formulation (cat# ab51253).

    IHC image of alpha Synuclein (phospho S129) staining in Human Parkinson Substantia Nigra formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab51253, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay performed on Human normal Substantia Nigra.

     For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Dot Blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] - BSA and Azide free (ab209422)
    Dot Blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] - BSA and Azide free (ab209422)

    Dot blot analysis of alpha Synuclein (pS129) peptide (Lane 1), alpha Synuclein (unmodified) peptide (Lane 2) labelling alpha Synuclein (pS129) with ab51253 at a dilution of 1/1000. Peroxidase conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51253).

  • ELISA - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] - BSA and Azide free (ab209422)
    ELISA - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] - BSA and Azide free (ab209422)

    This ELISA data was generated using the same anti-phospho alpha synuclein S129 antibody clone, EP1536Y, in a different buffer formulation (cat# ab51253).

    Direct ELISA antibody dose-response curve using ab51253. Antibody concentration of 0-5000 ng/mL. Antigen concentration of 1000 ng/mL. An alkaline phosphatase conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.

  • Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] - BSA and Azide free (ab209422)
    Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] - BSA and Azide free (ab209422)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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