Anti-Alpha-synuclein antibody [4D6] (ab1903)
![Anti-Alpha-synuclein antibody [4D6] (ab1903)](https://www.abcam.com/ps/products/1/ab1903/Images/ab1903-353713-AntiAlphasynuclein-phospho-S129-antibody-EP1536Y-westernblot-mouse-brain.jpg "Anti-Alpha-synuclein antibody [4D6] (ab1903)")
Key features and details
- Mouse monoclonal [4D6] to Alpha-synuclein
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Alpha-synuclein antibody [4D6]
See all Alpha-synuclein primary antibodies -
Description
Mouse monoclonal [4D6] to Alpha-synuclein -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Recombinant full length protein corresponding to Human Alpha-synuclein.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Ammonium sulfate precipitated and dialyzed tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
4D6 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-Alpha-synuclein antibody [4D6] (ab1903) This image is taken from Migdalska-Richards A. et al. Ann Neurol. 2016 Nov; 80(5): 766–775. Fig 5 doi: 10.1002/ana.24790
α-Synuclein (α-syn) and S129-phosphorylated α-synuclein protein levels in SNCA/SNCA mouse brains after 12 days of treatment with 4mM ambroxol (Amb). (A) Western blotting for α-synuclein (using ab1903) and serine 129 (S129)-phosphorylated α-synuclein protein (using ab51253) in the brainstem (example blots shown).
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![Western blot - Anti-Alpha-synuclein antibody [4D6] (ab1903)](https://www.abcam.com/ps/products/1/ab1903/Images/ab1903-5206-anti-alpha-synuclein-antibody-4d6-western-blot.jpg)
Western blot - Anti-Alpha-synuclein antibody [4D6] (ab1903)All lanes : Anti-Alpha-synuclein antibody [4D6] (ab1903) at 5 µg/ml
Lane 1 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
Lane 2 : SK N BE (Human neuroblastoma) Whole Cell Lysate
Lane 3 : SK N SH (Human neuroblastoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 16 kDa
Observed band size: 16 kDa
Additional bands at: 18 kDa (possible post-translational modification), 80 kDa (possible post-translational modification)
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [4D6] (ab1903)](https://www.abcam.com/ps/products/1/ab1903/Images/ab1903-5207-anti-alpha-synuclein-antibody-4d6-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [4D6] (ab1903)IHC-P using ab1903 showing lewy bodies in a human substantia nigra neuron(x400)
