Anti-Beta Arrestin 2 antibody (ab31294)
Key features and details
- Goat polyclonal to Beta Arrestin 2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Beta Arrestin 2 antibody
See all Beta Arrestin 2 primary antibodies -
Description
Goat polyclonal to Beta Arrestin 2 -
Host species
Goat -
Specificity
This antibody is expected to recognise both reported isoforms (NP_004304 and NP_945355). No cross reactivity is expected with Arrestin beta 1. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide:
C-HDHIPLPRPQS
, corresponding to Internal sequence amino acids 350-360 of Human Beta Arrestin 2.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Beta Arrestin 2 antibody (ab31294)Lane 1 : Anti-Beta Arrestin 2 antibody (ab31294) at 0.1 µg/ml
Lanes 2-3 : Anti-Beta Arrestin 2 antibody (ab31294) at 1 µg/ml
Lane 1 : human spleen whole cell lysate in RIPA buffer
Lane 2 : mouse brain whole cell lysate in RIPA buffer
Lane 3 : mouse spleen whole cell lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 46 kDa
Observed band size: 45-48 kDa why is the actual band size different from the predicted?Primary incubation: 1 hour at room temperature.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beta Arrestin 2 antibody (ab31294)Immunohistochemical analysis of paraffin-embedded Human cerebellum tissue, staining Beta Arrestin 2 with ab31294 at 4 µg/ml. Staining was detected using HRP. Antigen retrieval was by heat mediation with citrate buffer (pH 6).
