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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Regulators

Anti-Beta Arrestin 2 antibody (ab77208)

Anti-Beta Arrestin 2 antibody (ab77208)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal to Beta Arrestin 2
  • Suitable for: WB, ELISA, ICC/IF
  • Reacts with: Human, Recombinant fragment
  • Isotype: IgG2a

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Overview

  • Product name

    Anti-Beta Arrestin 2 antibody
    See all Beta Arrestin 2 primary antibodies
  • Description

    Mouse monoclonal to Beta Arrestin 2
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity

    Reacts with: Human, Recombinant fragment
  • Immunogen

    Recombinant fragment, corresponding to amino acids 300 - 410 of Human Beta Arrestin 2 (AAH07427), with a 26 kDa enzyme tag

  • Positive control

    • WB: HeLa cell lysate IF: HeLa cells
  • General notes

    This product was changed from ascites to tissue culture supernatant on 5th December 2019. Lot numbers higher than GR324089 are from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Constituent: 2.68% PBS
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Isotype

    IgG2a
  • Light chain type

    kappa
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Heterotrimeric G Proteins
    • Regulators

Images

  • Western blot - Anti-Beta Arrestin 2 antibody (ab77208)
    Western blot - Anti-Beta Arrestin 2 antibody (ab77208)
    Anti-Beta Arrestin 2 antibody (ab77208) at 1/500 dilution + recombinant tagged Beta Arrestin 2 (the immunogen) at 0.2 µg

    Secondary
    goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

    Developed using the ECL technique.

    Observed band size: 38 kDa
    why is the actual band size different from the predicted?



    This image was generated using the ascites version of the product.

  • Immunocytochemistry/ Immunofluorescence - Anti-Beta Arrestin 2 antibody (ab77208)
    Immunocytochemistry/ Immunofluorescence - Anti-Beta Arrestin 2 antibody (ab77208)

    Immunofluorescent analysis of HeLa cells using ab77208 at 10 ug/ml.

    This image was generated using the ascites version of the product.

  • ELISA - Anti-Beta Arrestin 2 antibody (ab77208)
    ELISA - Anti-Beta Arrestin 2 antibody (ab77208)

    Capture ELISA using ab77208 and recombinant tagged Beta Arrestin 2.

    This image was generated using the ascites version of the product.

  • Western blot - Anti-Beta Arrestin 2 antibody (ab77208)
    Western blot - Anti-Beta Arrestin 2 antibody (ab77208)
    Anti-Beta Arrestin 2 antibody (ab77208) at 1/500 dilution + HeLa cell lysate at 50 µg

    Secondary
    goat anti-mouse (H&L)-HRP conjugate at 1/2500 dilution

    Developed using the ECL technique.

    Observed band size: 46 kDa why is the actual band size different from the predicted?



    This image was generated using the ascites version of the product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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