Anti-Caspase-8 antibody (ab25901)
Key features and details
- Rabbit polyclonal to Caspase-8
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-Caspase-8 antibody
See all Caspase-8 primary antibodies -
Description
Rabbit polyclonal to Caspase-8 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
-
Immunogen
Synthetic peptide corresponding to Human Caspase-8 (C terminal). Synthetic peptide (Human) - a 16 amino acid peptide from near the carboxy-terminus of human Caspase-8 isoform A.
Database link: Q14790 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Caspase-8 Antibody is affinity chromatography purified via peptide column. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-Caspase-8 antibody (ab25901)Immunocytochemistry/Immunofluorescence of Jurkat cells labeling Caspase-8 with Anti-Caspase-8 antibody (ab25901) at 20μg/ml.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-8 antibody (ab25901)Immunohistochemistry of Caspase-8 in human spleen tissue with Caspase-8 antibody at 5 µg/ml.
-
Western blot - Anti-Caspase-8 antibody (ab25901)All lanes : Anti-Caspase-8 antibody (ab25901) at 0.5 µg/ml
Lane 1 : Human spleen
Lane 2 : Mouse spleen
Lane 3 : Rat spleen
Predicted band size: 55 kDa
-
Western blot - Anti-Caspase-8 antibody (ab25901)All lanes : Anti-Caspase-8 antibody (ab25901) at 1 µg/ml
Lane 1 : Jurkat cell lysates with Absence of blocking buffer
Lane 2 : Jurkat cell lysates with Presence of blocking buffer
Predicted band size: 55 kDa
-
Immunocytochemistry/ Immunofluorescence - Anti-Caspase-8 antibody (ab25901)ab25901 at 2ug/ml staining Caspase-8 in Jurkat cells by ICC/IF
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-8 antibody (ab25901)IHC image of Caspase-8 staining in human spleen formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab25901, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
