Human Caspase-9 ELISA Kit (ab119508)
Key features and details
- Sensitivity: 0.4 ng/ml
- Range: 1.6 ng/ml - 100 ng/ml
- Sample type: Cell culture supernatant, Cell Lysate, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Caspase-9 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 8 6.6% Inter-assay Sample n Mean SD CV% Overall 8 9% -
Sample type
Cell culture supernatant, Serum, Cell Lysate -
Assay type
Sandwich (quantitative) -
Sensitivity
0.4 ng/ml -
Range
1.6 ng/ml - 100 ng/ml -
Recovery
103 %
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Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Caspase-9 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human Caspase-9 concentrations in cell lysate, cell culture supernatant and serum.
Caspase-9 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and along with a Caspase-9 detection antibody and the microplate is then incubated at room temperature. Following washing a HRP conjugated anti-rabbit-IgG antibody is added to each well, incubated at room temperature then again washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Caspase-9 amount of sample captured in plate.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 20X Assay Buffer Concentrate 1 x 5ml 20X Wash Buffer Concentrate 1 x 50ml Adhesive Films 4 units Anti-rabbit-IgG HRP conjugated antibody 1 x 10µl Human Caspase-9 Standard 2 vials 10X Lysis buffer 1 x 15ml Microplate coated with monoclonal antibody to Human Caspase-9 (12 x 8 wells) 1 unit Polyclonal anti-Human Caspase-9 Detection Antibody (rabbit) 1 x 70µl Sample Diluent 1 x 12ml Stop Solution (1M Phosphoric acid) 1 x 15ml TMB Substrate Solution 1 x 15ml -
Research areas
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Function
Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9. -
Tissue specificity
Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes. -
Sequence similarities
Belongs to the peptidase C14A family.
Contains 1 CARD domain. -
Developmental stage
Expressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart. -
Post-translational
modificationsCleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events. - Information by UniProt
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Alternative names
- APAF-3
- APAF3
- Apoptosis related cysteine peptidase
see all -
Database links
- Entrez Gene: 842 Human
- Omim: 602234 Human
- SwissProt: P55211 Human
- Unigene: 329502 Human
Images
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Typical Standard Curve
Representative Standard Curve using ab119508.
