Antibodies, Proteins, Kits and Reagents for Life Science

351 792 ₸ Product size

100 µl 351 792 ₸

Order now and get it on Wednesday March 03, 2021

Anti-ErbB4 / HER4 antibody [E200] (ab32375)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [E200] to ErbB4 / HER4
Suitable for: WB, IP
Reacts with: Human

Product name

Anti-ErbB4 / HER4 antibody [E200]
See all ErbB4 / HER4 primary antibodies
Description

Rabbit monoclonal [E200] to ErbB4 / HER4
Host species

Rabbit
Specificity

This antibody is specific to ErbB4 / HER4. It does not cross react with other EGF receptor family members.
Tested Applications & Species

Application Species
IP
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human ErbB4/ HER4 (C terminal). The exact sequence is proprietary.
Positive control
- WB: MCF7 and T47D cell lysate. IP: HEK-293 cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Clonality

Monoclonal
Clone number

E200
Isotype

IgG
Research areas

Western blot - Anti-ErbB4 / HER4 antibody [E200] (ab32375)

All lanes : Anti-ErbB4 / HER4 antibody [E200] (ab32375) at 1.09 µg/ml

Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell)
Lane 2 : T47D (Human ductal breast epithelial tumor epithelial cell)
Lane 3 : T47D (Human ductal breast epithelial tumor epithelial cell) low exposure image of lane 2

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml

Predicted band size: 147 kDa
Western blot - Anti-ErbB4 / HER4 antibody [E200] (ab32375)

Anti-ErbB4 / HER4 antibody [E200] (ab32375) at 1/4000 dilution + MCF7 cell lysate

Predicted band size: 147 kDa
Observed band size: 185 kDa
why is the actual band size different from the predicted?
Immunoprecipitation - Anti-ErbB4 / HER4 antibody [E200] (ab32375)

Lane 1 (input): HEK-293 (human embryonic kidney epithelial cell) whole cell lysate, 10μg
Lane 2 (+): HEK-293 whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32375 in HEK-293 whole cell lysate

Ab32375 Immunoprecipitating ErbB 4 in HEK-293 whole cell lysate. Capture antibody was used at a 1:50 dilution (2μg in 0.35mg lysates). For western blotting, primary antibody used as ab32375 at 1:500 dilution. Ab131366 VeriBlot for IP Detection Reagent (HRP) was used for detection at 1:1000 dilution. The lower band at around 75kDa should be proteolysis fragment based on the literature. (PMID: 9362517)

Blocking and diluting buffer: 5% NFDM/TBST
Anti-ErbB4 / HER4 antibody [E200] (ab32375)

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