Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ErbB4 / HER4 (phospho Y1284) antibody [EPR2273(2)] - BSA and Azide free (ab232527)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2273(2)] to ErbB4 / HER4 (phospho Y1284) - BSA and Azide free
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ErbB4 / HER4 (phospho Y1284) antibody [EPR2273(2)] - BSA and Azide free
    See all ErbB4 / HER4 primary antibodies

  • Description

    Rabbit monoclonal [EPR2273(2)] to ErbB4 / HER4 (phospho Y1284) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody detects ErbB4 / HER4 phosphorylated at Tyrosine 1284 and may also detect ErbB2 / HER2 phosphorylated at Tyrosine 1248.

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: A431 cells.

  • General notes

    Ab232527 is the carrier-free version of ab109273. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab232527 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-ErbB4 / HER4 (phospho Y1284) antibody [EPR2273(2)] - BSA and Azide free (ab232527)
    Flow Cytometry - Anti-ErbB4 / HER4 (phospho Y1284) antibody [EPR2273(2)] - BSA and Azide free (ab232527)

    ab109273 at 1/10 dilution staining ErbB4 / HER4 in HeLa cells by Flow cytometry.
    Green: Permeabilized A431 cells, untreated
    Red: Permeabilized A431 cells, treated with EGF.
    Blue: Permeabilized A431 cells, treated with EGF and preincubated with phospho-ErbB4 / HER4 (pY1284) peptide
    Orange: Permeabilized A431 cells, treated with EGF and preincubated with non-phospho-ErbB4 / HER4(pY1284) peptide.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109273).

  • Immunocytochemistry/ Immunofluorescence - Anti-ErbB4 / HER4 (phospho Y1284) antibody [EPR2273(2)] - BSA and Azide free (ab232527)
    Immunocytochemistry/ Immunofluorescence - Anti-ErbB4 / HER4 (phospho Y1284) antibody [EPR2273(2)] - BSA and Azide free (ab232527)

    Immunofluorescent analysis of A431 (human epidermoid carcinoma cell line) cells labeling ErbB4 / HER4 (phospho Y1284) using ab232527 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109273).

  • Anti-ErbB4 / HER4 (phospho Y1284) antibody [EPR2273(2)] - BSA and Azide free (ab232527)
    Anti-ErbB4 / HER4 (phospho Y1284) antibody [EPR2273(2)] - BSA and Azide free (ab232527)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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