Anti-ErbB4 / HER4 (phospho Y1284) antibody [EPR2273(2)] - BSA and Azide free (ab232527)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2273(2)] to ErbB4 / HER4 (phospho Y1284) - BSA and Azide free
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-ErbB4 / HER4 (phospho Y1284) antibody [EPR2273(2)] - BSA and Azide free
See all ErbB4 / HER4 primary antibodies -
Description
Rabbit monoclonal [EPR2273(2)] to ErbB4 / HER4 (phospho Y1284) - BSA and Azide free -
Host species
Rabbit -
Specificity
This antibody detects ErbB4 / HER4 phosphorylated at Tyrosine 1284 and may also detect ErbB2 / HER2 phosphorylated at Tyrosine 1248.
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Tested applications
Suitable for: WB, ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: A431 cells.
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General notes
Ab232527 is the carrier-free version of ab109273. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab232527 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.20
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2273(2) -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-ErbB4 / HER4 (phospho Y1284) antibody [EPR2273(2)] - BSA and Azide free (ab232527)
ab109273 at 1/10 dilution staining ErbB4 / HER4 in HeLa cells by Flow cytometry.
Green: Permeabilized A431 cells, untreated
Red: Permeabilized A431 cells, treated with EGF.
Blue: Permeabilized A431 cells, treated with EGF and preincubated with phospho-ErbB4 / HER4 (pY1284) peptide
Orange: Permeabilized A431 cells, treated with EGF and preincubated with non-phospho-ErbB4 / HER4(pY1284) peptide.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109273).
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Immunocytochemistry/ Immunofluorescence - Anti-ErbB4 / HER4 (phospho Y1284) antibody [EPR2273(2)] - BSA and Azide free (ab232527)
Immunofluorescent analysis of A431 (human epidermoid carcinoma cell line) cells labeling ErbB4 / HER4 (phospho Y1284) using ab232527 at 1/100 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109273).
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