Antibodies, Proteins, Kits and Reagents for Life Science

251 280 ₸ Product size

100 µl 251 280 ₸

Order now and get it on Tuesday March 02, 2021

Anti-ErbB4 / HER4 (phospho Y1284) antibody [EPR2273(2)] (ab109273)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR2273(2)] to ErbB4 / HER4 (phospho Y1284)
Suitable for: WB, Flow Cyt, ICC/IF
Reacts with: Human

Product name

Anti-ErbB4 / HER4 (phospho Y1284) antibody [EPR2273(2)]
See all ErbB4 / HER4 primary antibodies
Description

Rabbit monoclonal [EPR2273(2)] to ErbB4 / HER4 (phospho Y1284)
Host species

Rabbit
Specificity

This antibody detects ErbB4 / HER4 phosphorylated at Tyrosine 1284 and may also detect Erbb2/HER2 phosphorylated at Tyrosine 1248.

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
WB	Human

See all applications and species data

Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- A431 cell lysate, treated with Epidermal Growth Factor (EGF); A431 cells; permeabilized EGF-treated A431 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR2273(2)
Isotype

IgG
Research areas

Western blot - Anti-ErbB4 / HER4 (phospho Y1284) antibody [EPR2273(2)] (ab109273)

All lanes : Anti-ErbB4 / HER4 (phospho Y1284) antibody [EPR2273(2)] (ab109273) at 1/1000 dilution

Lane 1 : A431 cell lysate, untreated
Lane 2 : A431 cell lysate, treated with Epidermal Growth Factor (EGF)

Lysates/proteins at 10 µg/ml per lane.

Predicted band size: 147 kDa
Flow Cytometry - Anti-ErbB4 / HER4 (phospho Y1284) antibody [EPR2273(2)] (ab109273)

ab109273 at 1/10 dilution staining ErbB 4 in HeLa cells by Flow cytometry.
Green: Permeabilized A431 cells, untreated
Red: Permeabilized A431 cells, treated with EGF.
Blue: Permeabilized A431 cells, treated with EGF and preincubated with phospho-ErbB 4 (pY1284) peptide
Orange: Permeabilized A431 cells, treated with EGF and preincubated with non-phospho-ErbB 4(pY1284) peptide.
Immunocytochemistry/ Immunofluorescence - Anti-ErbB4 / HER4 (phospho Y1284) antibody [EPR2273(2)] (ab109273)

Immunofluorescent staining of A431 cells using ab109273 at a dilution of 1/100.
Anti-ErbB4 / HER4 (phospho Y1284) antibody [EPR2273(2)] (ab109273)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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