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Anti-CD22 antibody [OX97] - BSA and Azide free (ab244594)

Key features and details

  • Rat monoclonal [OX97] to CD22 - BSA and Azide free
  • Suitable for: IHC-Fr, Flow Cyt
  • Reacts with: Mouse
  • Isotype: IgG1

Overview

  • Product name

    Anti-CD22 antibody [OX97] - BSA and Azide free
    See all CD22 primary antibodies

  • Description

    Rat monoclonal [OX97] to CD22 - BSA and Azide free

  • Host species

    Rat

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    IHC-Fr
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant full length protein corresponding to Mouse CD22.

  • Positive control

    • Flow Cyt: C57BL/6 mouse splenocytes. IHC-Fr: Mouse spleen tissue.

  • General notes

    ab244594 is the carrier free version of ab243840.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

Properties

Images

  • Immunohistochemistry (Frozen sections) - Anti-CD22 antibody [OX97] - BSA and Azide free (ab244594)
    Immunohistochemistry (Frozen sections) - Anti-CD22 antibody [OX97] - BSA and Azide free (ab244594)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab243840).

    IHC image of CD22 staining in a section of frozen normal mouse spleen. 

    The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M glycine and 1% (w/v) BSA for 1h at room temperature.  The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab243840 at 1µg/ml and ab183685 (Rabbit monoclonal [EPR19514] to CD4) at 1/200, to show the distinct staining of B cells and T cells. The section was then incubated with ab150165 (Goat Anti-Rat IgG H&L (Alexa Fluor® 488) preabsorbed, (Shown in green) 1/1000) and ab150080 (Goat Anti-Rabbit IgG H&L (Alexa Fluor®594) (Shown in red) 1/1000) for 1 hour at room temperature. The secondary-only control insert image is taken from an identical assay without primary antibody. DAPI was used to stain the cell nuclei (blue). The section was then mounted using Fluoromount®.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    For IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antibody concentrations and incubation times. 

  • Flow Cytometry - Anti-CD22 antibody [OX97] - BSA and Azide free (ab244594)
    Flow Cytometry - Anti-CD22 antibody [OX97] - BSA and Azide free (ab244594)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab243840).

    C57BL/6 mouse splenocytes stained with ab243840 (right) or Rat IgG1κ (ab18407) isotype (left). C57BL/6 mouse splenocytes were incubated for 30 min on ice in 1x PBS / 10 % mouse serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab243840) or Rat IgG1κ (ab18407) (1x106 in 100µl at 0.2 µg/ml) for 30 min on ice.

    The secondary antibody Goat anti-rat IgG H&L (Alexa Fluor ® 488, pre-adsorbed) (ab150165) was used at 1/2000 dilution for 30 min at 4°C.

    Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable lymphocytes.

     

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