Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD80 antibody [EPR22183] - BSA and Azide free (ab238648)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22183] to CD80 - BSA and Azide free
  • Suitable for: ICC/IF, Flow Cyt, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-CD80 antibody [EPR22183] - BSA and Azide free
    See all CD80 primary antibodies

  • Description

    Rabbit monoclonal [EPR22183] to CD80 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: Raji cells.

  • General notes

    Ab238648 is the carrier-free version of ab225674. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab238648 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-CD80 antibody [EPR22183] - BSA and Azide free (ab238648)
    Flow Cytometry - Anti-CD80 antibody [EPR22183] - BSA and Azide free (ab238648)

    Flow cytometric analysis of HUVEC (human umbilical vein endothelial cell line) (left panel) cells and Raji (human Burkitt's lymphoma cell line)(right panel) cells labeling CD80  with ab225674 at 1/400 (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

    Gated on viable cells.

    Negative control: HUVEC (PMID: 15064090).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225674).

  • Immunoprecipitation - Anti-CD80 antibody [EPR22183] - BSA and Azide free (ab238648)
    Immunoprecipitation - Anti-CD80 antibody [EPR22183] - BSA and Azide free (ab238648)

    CD80 was immunoprecipitated from 0.35 mg of Raji (human Burkitt's lymphoma cell line) whole cell lysate with ab225674 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab225674 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: Raji whole cell lysate 10 μg (Input).
    Lane 2: ab225674 IP in Raji whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab225674 in Raji whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 1 second.

    This blot was developed using a higher sensitivity ECL substrate.

     

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225674).

  • Immunocytochemistry/ Immunofluorescence - Anti-CD80 antibody [EPR22183] - BSA and Azide free (ab238648)
    Immunocytochemistry/ Immunofluorescence - Anti-CD80 antibody [EPR22183] - BSA and Azide free (ab238648)

    Immunofluorescent analysis of 100% methanol-fixed Raji (human Burkitt's lymphoma cell line) and HUVEC (human umbilical vein endothelial cell line) cells labeling CD80 with ab225674 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in Raji cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

    PBS only control: Used PBS instead of primary antibody, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    Negative control: HUVEC cells (PMID: 15064090).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225674).

  • Anti-CD80 antibody [EPR22183] - BSA and Azide free (ab238648)
    Anti-CD80 antibody [EPR22183] - BSA and Azide free (ab238648)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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