Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MS4A2 antibody [EPR24324-7] - BSA and Azide free (ab280570)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24324-7] to MS4A2 - BSA and Azide free
  • Suitable for: Dot blot, ICC, IP, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-MS4A2 antibody [EPR24324-7] - BSA and Azide free
    See all MS4A2 primary antibodies

  • Description

    Rabbit monoclonal [EPR24324-7] to MS4A2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Dot blot, ICC, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt (Intra) or WB

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human colon, breast carcinoma and prostatic hyperplasia tissue. ICC: HEK-293T cells. IP: HEK-293T cell. Dot Blot: Human MS4A2 peptide.

  • General notes

    ab280570 is the carrier-free version of ab271068. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab280570 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Constituent: 100% PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR24324-7

  • Isotype

    IgG

  • Research areas

Images

  • Dot Blot - Anti-MS4A2 antibody [EPR24324-7] - BSA and Azide free (ab280570)
    Dot Blot - Anti-MS4A2 antibody [EPR24324-7] - BSA and Azide free (ab280570)

    This data was developed using ab271068, the same antibody clone in a different buffer formulation.

    Dot blot analysis of MS4A2 using ab271068 at 1:1000 dilution followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1:100,000 dilution.

    Lane 1: Human MS4A2 peptide (aa 226-243)
    Lane 2: Human MS4A2 peptide (aa 206-223)
    Lane 3: Human MS4A2 peptide (aa 151-171)

    Exposure time: 3 minutes

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MS4A2 antibody [EPR24324-7] - BSA and Azide free (ab280570)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MS4A2 antibody [EPR24324-7] - BSA and Azide free (ab280570)

    This data was developed using ab271068, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human colon tissue labelling MS4A2 with ab271068 at 1/2000 dilution (0.221 µg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on mast cells of human colon. The section was incubated with ab271068 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunocytochemistry - Anti-MS4A2 antibody [EPR24324-7] - BSA and Azide free (ab280570)
    Immunocytochemistry - Anti-MS4A2 antibody [EPR24324-7] - BSA and Azide free (ab280570)

    This data was developed using ab271068, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T cells labelling MS4A2 with ab271068 at 1/500 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) antibody at 1/500 dilution (Red).

    Confocal image showing cytoplasmic staining in HEK-293T cells transfected with myc-tagged MS4A2 expression vector (PMID:18571665).

    ab202008 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 488) was used to counterstain tubulin at 1/100 dilution (Green). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/500 dilution.

  • Immunoprecipitation - Anti-MS4A2 antibody [EPR24324-7] - BSA and Azide free (ab280570)
    Immunoprecipitation - Anti-MS4A2 antibody [EPR24324-7] - BSA and Azide free (ab280570)

    This data was developed using ab271068, the same antibody clone in a different buffer formulation.

    MS4A2 was immunoprecipitated from 0.35 mg HEK-293T (human embryonic kidney) transfected with MS4A2 expression vector, whole cell lysate 10 µg with ab271068 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab271068 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: HEK-293T transfected with MS4A2 expression vector, whole cell lysate 10 µg

    Lane 2: ab271068 IP in HEK-293T transfected with MS4A2 expression vector, whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab271068 in HEK-293T transfected with MS4A2 expression vector, whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 7.75 seconds

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MS4A2 antibody [EPR24324-7] - BSA and Azide free (ab280570)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MS4A2 antibody [EPR24324-7] - BSA and Azide free (ab280570)

    This data was developed using ab271068, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labelling MS4A2 with ab271068 at 1/2000 dilution (0.221 µg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on mast cells of human breast carcinoma. The section was incubated with ab271068 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MS4A2 antibody [EPR24324-7] - BSA and Azide free (ab280570)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MS4A2 antibody [EPR24324-7] - BSA and Azide free (ab280570)

    This data was developed using ab271068, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human prostatic hyperplasia tissue labelling MS4A2 with ab271068 at 1/2000 dilution (0.221 µg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on mast cells of human prostatic hyperplasia. The section was incubated with ab271068 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-MS4A2 antibody [EPR24324-7] - BSA and Azide free (ab280570)
    Anti-MS4A2 antibody [EPR24324-7] - BSA and Azide free (ab280570)

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