Anti-pan CEACAM antibody [D14HD11] (ab4567)
Key features and details
- Mouse monoclonal [D14HD11] to pan CEACAM
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-pan CEACAM antibody [D14HD11]
See all pan CEACAM primary antibodies -
Description
Mouse monoclonal [D14HD11] to pan CEACAM -
Host species
Mouse -
Specificity
Broadly reactive with high affinity (affinity to solid phase bound CEACAM 5: 2 x 1010 L/Mol [Jantscheff P et al.]) with most of the CEACAM molecules (except CEACAM 7 and CEACAM 8) transiently expressed on the cell surface of transfected BOSC23 cells. This antibody can be used to detect CEACAM 1 (BGP/CD66a), CEACAM 3 (CGM1/CD66d), CEACAM 4 (CGM 7), CEACAM 5 and CEACAM 6 (NCA/CD66c) on transfectants by flow cytometry. -
Species reactivity
Reacts with: Human -
Immunogen
Full length native Carcino Embryonic Antigen (partially purified) (Human) from a perchloric acid extract from liver metastases of colonic tumors (Jantscheff P et al.).
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General notes
Antibodies produced from cDNA: Conventional technologies usually either generate antibodies against purified proteins, or against synthetic peptides based on amino acid sequences derived from DNA sequence data. Genetic immunization involves introducing the gene in the form of a cDNA directly into an animal which translates this cDNA into protein thus stimulating an immune response against the foreign protein. Although the synthetic peptide approach is comparable in speed, the quality of antibodies generated by genetic immunization is far superior. This is because the protein is made by the immunized animal, utilzing complex cellular mechanisms that allow it to gain a native conformation. Antibodies are then generated against a native protein, such as is found in the blood or tissues of its host species. Membrane-bound or secreted proteins often create problems for conventional antibody technology because in their native form, they are often modified by glycosylation, or in some cases exist as multiple membrane-spanning proteins that are not soluble following isolation or synthesis in recombinant systems. All of these problems are avoided if the immunized animal makes the protein itself. Antibodies generated by genetic immunization have been shown to have binding affinities to the protein in the sub-nanomolar range, which are approximately 100x higher than conventionally developed antibodies and much higher than single chain antibodies. Results confirm published data for much higher avidity of sera generated by genetic immunization as compared with that gained by immunization with a corresponding recombinant protein.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
D14HD11 -
Isotype
IgG1 -
Research areas
