Anti-CD80 antibody [EPR22127-317] - BSA and Azide free (ab238650)
![Anti-CD80 antibody [EPR22127-317] - BSA and Azide free (ab238650)](https://www.abcam.com/ps/products/238/ab238650/Images/ab238650-316635-anti-cd80-antibody-epr22127-317-western-blot.jpg "Anti-CD80 antibody [EPR22127-317] - BSA and Azide free (ab238650)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22127-317] to CD80 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse
Overview
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Product name
Anti-CD80 antibody [EPR22127-317] - BSA and Azide free
See all CD80 primary antibodies -
Description
Rabbit monoclonal [EPR22127-317] to CD80 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: C2C12 and DC2.4 whole cell lysates.
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General notes
Ab238650 is the carrier-free version of ab238481. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab238650 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22127-317 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CD80 antibody [EPR22127-317] - BSA and Azide free (ab238650)All lanes : Anti-CD80 antibody [EPR22127-317] (ab238481) at 1/1000 dilution
Lane 1 : C2C12 (mouse myoblast cell line) whole cell lysate
Lane 2 : DC2.4 (mouse dendritic cell line) whole cell lysate
Lane 3 : P815 (mouse mastocytoma mast cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 35 kDa
Observed band size: 55-60 kDa why is the actual band size different from the predicted?
Exposure time: 26 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The molecular mass observed is consistent with what has been described in the literature (PMID: 7544014, PMID: 9079798); 55-60 kDa is due to glycosylation (PMID: 9654121).
Negative control: P815 (PMID: 9237108).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238481).
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![Anti-CD80 antibody [EPR22127-317] - BSA and Azide free (ab238650)](https://www.abcam.com/ps/products/238/ab238650/Images/ab238650-2-benefits-of-recombinant-antibodies.png)
Anti-CD80 antibody [EPR22127-317] - BSA and Azide free (ab238650)
