Flow cytometry analysis of CD26 in PC12 cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a CD26 monoclonal antibody (ab119346) at a dilution of 2 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.

Flow cytometry analysis of CD26 in Hela cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a CD26 monoclonal antibody (ab119346) at a dilution of 2 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.

ab119346 labelling CD26 (green) in the cytoplasm of HeLa cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:20 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated Goat anti-mouse IgG (H+L) was used as the secondary antibody. Red (phalloidin) - F-actin, Blue (DAPI) - nuclei. Images were taken at a magnification of 60x.

ab119346 labelling CD26 (green) in the cytoplasm of PC12 cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:20 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated Goat anti-mouse IgG (H+L) was used as the secondary antibody. Red (phalloidin) - F-actin, Blue (DAPI) - nuclei. Images were taken at a magnification of 60x.

ab119346 labelling CD26 (green) in the cytoplasm of H-4-II-E cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:20 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated Goat anti-mouse IgG (H+L) was used as the secondary antibody. Red (phalloidin) - F-actin, Blue (DAPI) - nuclei. Images were taken at a magnification of 60x.